المهنية في صناعة الخرز المغناطيسي الحيوي
استخراج الخرز المغناطيسي يدوياً في الشتاء: الاحتياطات الرئيسية للتعامل مع درجات الحرارة المنخفضة
مقدمة
When manually using magnetic bead – based nucleic acid extraction reagents in cold winter, special attention should be paid to the impact of temperature on reagent activity, magnetic bead adsorption efficiency, and experimental operations. The specific precautions are as follows:
1. Reagent Rewarming in Advance
Reagents such as magnetic bead suspensions, lysis buffers, and washing buffers are prone to component precipitation or magnetic bead aggregation at low temperatures. Take them out of the refrigerator 15 – 30 minutes in advance, let them rewarm at room temperature, and gently invert and mix them. Avoid violent shaking to prevent magnetic beads from breaking.
2. Maintain a Stable Operating Environment Temperature
Try to operate in a laboratory with a room temperature of 18 – 25درجه مئوية. Avoid areas directly blown by cold air, مثل الهواء – conditioner outlets and doors and windows. If the ambient temperature is too low, use a constant – temperature metal bath or water bath to maintain the temperature of samples and reagents, preventing a decrease in the efficiency of the lysis buffer and insufficient binding between DNA and magnetic beads.
3. Control Time in Magnetic Bead Adsorption and Separation Steps
Low temperatures will slow down the binding speed of magnetic beads and nucleic acids. Appropriately extend the incubation time of magnetic beads (usually increase by 5 – 10 دقائق). في نفس الوقت, when separating magnetic beads on the magnetic stand, the sedimentation speed of magnetic beads slows down at low temperatures. Prolong the standing time to ensure that the magnetic beads are completely adsorbed on the tube wall before discarding the waste liquid to avoid the loss of magnetic beads.
4. Optimize the Temperature of the Eluent
The temperature of the eluent directly affects the nucleic acid elution efficiency. In winter, pre – warm the eluent to 50 – 65℃ before use. Briefly vortex and mix during elution and let it stand for 2 – 3 minutes to improve the nucleic acid recovery rate. The eluted nucleic acid sample should be stored at a low temperature as soon as possible to prevent degradation.
5. Operational Details and Consumable Preparation
Pre – warm the pipette in advance to avoid inaccurate liquid aspiration by the pipette at low temperatures. Use enzyme – free centrifuge tubes and pipette tips to prevent the loss of nucleic acids adsorbed on the surface of consumables in a low – temperature environment. Operate gently to reduce reagent volatilization and sample contamination.
مزود
شركة شنغهاي لينغجون للتكنولوجيا الحيوية, المحدودة.تأسست في 2016 وهي شركة متخصصة في تصنيع المواد المغناطيسية الحيوية وكواشف استخلاص الحمض النووي.
لدينا خبرة غنية في استخراج وتنقية الحمض النووي, تنقية البروتين, فصل الخلايا, التألق الكيميائي, وغيرها من المجالات التقنية.
منتجاتنا تستخدم على نطاق واسع في العديد من المجالات, مثل الفحوصات الطبية, الاختبارات الجينية, البحوث الجامعية, التكاثر الوراثي, وهكذا. نحن لا نقدم المنتجات فحسب، بل يمكننا أيضًا إجراء تصنيع المعدات الأصلية, أوديإم, وغيرها من الاحتياجات. إذا كان لديك حاجة ذات صلة, لا تتردد في الاتصال بنا .
