كيف الخرز المغناطيسي القائم على السيليكا 220012 تحسين فرز الأجزاء

مقدمة

في أبحاث البيولوجيا الجزيئية, يعد فرز وتنقية أجزاء الحمض النووي رابطًا رئيسيًا للحصول على بيانات تسلسل عالية الجودة. غالبًا ما تواجه الطرق التقليدية مشاكل مثل التشغيل المرهق, low efficiency and unstable recovery rate. ال 220012 الخرز المغناطيسي القائم على السيليكا introduced by Shanghai Lingjun Biotechnology Co., المحدودة. provide an efficient and reliable solution to this problem.

1. Small size, high performance

ال 220012 magnetic beads were designed with the high standards of fragment sorting in mind. Its average particle size of 400 nm gives the beads a large specific surface area, which significantly increases the binding capacity of small nucleic acid fragments.

The modified silicone hydroxyl group (سي-أوه) on the surface of the beads enables efficient and specific adsorption of nucleic acids, ensuring precise capture of target fragments in complex sample matrices.

The monodisperse nature of the beads ensures homogeneity in the suspension, resulting in excellent reproducibility of the results of each sorting operation, which lays a solid foundation for the stability of the experimental data.

2.Experimental investigation, results demonstration

A certain concentration of binding solution containing NaCl and PEG was prepared and mixed with 220012 magnetic beads to form a magnetic bead suspension, 100 μl of sample (containing a total amount of 1 μg of dye-free DNA Marker with a size of 100-5,000 بي بي) was added, and the first round was added with 2×-0.45× volume of sorting magnetic beads for DNA fragment purification, respectively. In the second round, 0.15-0.2× volume of sorting magnetic beads were added, and the sorted products were detected by electrophoresis using a 1.2% هلام الاغاروز.

The unilateral recovery of DNA fragments is shown in the table below:

الخرز:الحمض النووي	≥1.2×	1-0.8×	0.7-0.6×	0.58-0.5×	0.5×	0.45×
DNA sorting	≥100bp	≥200bp	≥300bp	≥400bp	≥500bp	＞1000bp

Bilateral recovery of DNA fragments is shown in the table below:

First Volume Ratio （Beads:DNA）	0.8×	0.7×	0.6×	0.58×	0.5×	0.45×
Second Volume Ratio （Beads:DNA）	0.2×	0.2×	0.2×	0.15×	0.15×	0.15×
DNA sorting	200-400بي بي	300-500بي بي	300-600بي بي	300-700بي بي	300-1000بي بي	400-2000بي بي

3. Precise sorting, wide range of applications

The core application of 220012 magnetic beads is the precise sorting and purification of DNA fragments. It can efficiently purify DNA fragments in the recovery range of 100 bp to 50 كيلو بايت.

In the field of NGS sequencing, the beads have excellent performance and are suitable for PCR product purification, sequencing reaction product purification and the key DNA fragment sorting step, which can effectively remove primer dimers and other interfering substances to improve the quality of library construction.

Especially in the research of circulating free DNA (cfDNA) and circulating tumour DNA (ctDNA), على سبيل المثال, in the fields of prenatal diagnosis and early tumour screening, ال 220012 magnetic beads can effectively enrich trace amounts of target nucleic acids in serum/plasma and provide high-purity templates for downstream analysis.

4. Simple handling, multiplying efficiency

Compared with the traditional agarose gel recovery method, 220012 magnetic beads can be operated without centrifugation, electrophoresis or exposure to toxic reagents, which greatly simplifies the experimental steps.

The excellent suspension properties (half-settling time > 1 ساعة) make the beads easy to mix and manipulate in solution, reducing experimental errors caused by uneven settling.

The beads have good magnetic response (magnetic saturation strength ≥50 emu/g), can be quickly separated under the action of the magnetic field, shorten the waiting time for experiments, and easily adapted to automated liquid workstations to achieve high-throughput sample processing, which significantly improves the efficiency of laboratory work.

5. Stable, reliable and trustworthy

As a high-tech enterprise that has passed many international quality management system certifications, Shanghai Lingjun Biotechnology produces 220012 magnetic beads with small batch-to-batch variation and stable quality.

Strict quality control standards ensure that the performance of each batch of magnetic beads is highly consistent, so that the data of experimental projects carried out at different times are comparable, providing a guarantee for the continuity and reliability of scientific research work.

The magnetic beads are available in a variety of packaging sizes (from 2mL to 1000mL), which can meet the needs of both small-scale trials in scientific research and large-scale industrial production.

مزود

شركة شنغهاي لينغجون للتكنولوجيا الحيوية, المحدودة.تأسست في 2016 وهي شركة متخصصة في تصنيع المواد المغناطيسية الحيوية وكواشف استخلاص الحمض النووي.

لدينا خبرة غنية في استخراج وتنقية الحمض النووي, تنقية البروتين, فصل الخلايا, التألق الكيميائي, وغيرها من المجالات التقنية.

منتجاتنا تستخدم على نطاق واسع في العديد من المجالات, مثل الفحوصات الطبية, الاختبارات الجينية, البحوث الجامعية, التكاثر الوراثي, وهكذا. نحن لا نقدم المنتجات فحسب، بل يمكننا أيضًا إجراء تصنيع المعدات الأصلية, أوديإم, وغيرها من الاحتياجات. إذا كان لديك حاجة ذات صلة, لا تتردد في الاتصال بنا .