실리카 기반 자기 비드가 어떻게 만들어지는가? 220012 조각 정렬 개선

소개

In molecular biology research, DNA 단편의 분류 및 정제는 고품질 염기서열 분석 데이터를 얻기 위한 핵심 링크입니다.. 기존 방식은 번거로운 작업 등의 문제에 직면하는 경우가 많습니다., low efficiency and unstable recovery rate. 그만큼 220012 실리카 기반 자기 비드 introduced by Shanghai Lingjun Biotechnology Co., 주식회사. 이 문제에 대한 효율적이고 안정적인 솔루션을 제공합니다..

1. Small size, high performance

그만큼 220012 magnetic beads were designed with the high standards of fragment sorting in mind. Its average particle size of 400 nm gives the beads a large specific surface area, which significantly increases the binding capacity of small nucleic acid fragments.

The modified silicone hydroxyl group (시오) on the surface of the beads enables efficient and specific adsorption of nucleic acids, ensuring precise capture of target fragments in complex sample matrices.

The monodisperse nature of the beads ensures homogeneity in the suspension, resulting in excellent reproducibility of the results of each sorting operation, which lays a solid foundation for the stability of the experimental data.

2.Experimental investigation, results demonstration

A certain concentration of binding solution containing NaCl and PEG was prepared and mixed with 220012 magnetic beads to form a magnetic bead suspension, 100 μl of sample (containing a total amount of 1 μg of dye-free DNA Marker with a size of 100-5,000 bp) was added, and the first round was added with 2×-0.45× volume of sorting magnetic beads for DNA fragment purification, 각기. In the second round, 0.15-0.2× volume of sorting magnetic beads were added, and the sorted products were detected by electrophoresis using a 1.2% agarose gel.

The unilateral recovery of DNA fragments is shown in the table below:

염주:DNA	≥1.2×	1-0.8×	0.7-0.6×	0.58-0.5×	0.5×	0.45×
DNA sorting	≥100bp	≥200bp	≥300bp	≥400bp	≥500bp	＞1000bp

Bilateral recovery of DNA fragments is shown in the table below:

First Volume Ratio （Beads:DNA）	0.8×	0.7×	0.6×	0.58×	0.5×	0.45×
Second Volume Ratio （Beads:DNA）	0.2×	0.2×	0.2×	0.15×	0.15×	0.15×
DNA sorting	200-400bp	300-500bp	300-600bp	300-700bp	300-1000bp	400-2000bp

3. Precise sorting, wide range of applications

The core application of 220012 magnetic beads is the precise sorting and purification of DNA fragments. It can efficiently purify DNA fragments in the recovery range of 100 bp to 50 kb.

In the field of NGS sequencing, the beads have excellent performance and are suitable for PCR product purification, sequencing reaction product purification and the key DNA fragment sorting step, which can effectively remove primer dimers and other interfering substances to improve the quality of library construction.

Especially in the research of circulating free DNA (cfDNA) and circulating tumour DNA (ctDNA), 예를 들어, in the fields of prenatal diagnosis and early tumour screening, 그만큼 220012 magnetic beads can effectively enrich trace amounts of target nucleic acids in serum/plasma and provide high-purity templates for downstream analysis.

4. Simple handling, multiplying efficiency

Compared with the traditional agarose gel recovery method, 220012 magnetic beads can be operated without centrifugation, electrophoresis or exposure to toxic reagents, which greatly simplifies the experimental steps.

The excellent suspension properties (half-settling time > 1 시간) make the beads easy to mix and manipulate in solution, reducing experimental errors caused by uneven settling.

The beads have good magnetic response (magnetic saturation strength ≥50 emu/g), can be quickly separated under the action of the magnetic field, shorten the waiting time for experiments, and easily adapted to automated liquid workstations to achieve high-throughput sample processing, which significantly improves the efficiency of laboratory work.

5. Stable, reliable and trustworthy

As a high-tech enterprise that has passed many international quality management system certifications, Shanghai Lingjun Biotechnology produces 220012 magnetic beads with small batch-to-batch variation and stable quality.

Strict quality control standards ensure that the performance of each batch of magnetic beads is highly consistent, so that the data of experimental projects carried out at different times are comparable, providing a guarantee for the continuity and reliability of scientific research work.

The magnetic beads are available in a variety of packaging sizes (from 2mL to 1000mL), which can meet the needs of both small-scale trials in scientific research and large-scale industrial production.

공급자

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광, 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련된 필요사항이 있는 경우, 저희에게 연락하게 자유롭게 느끼십시오 .