シリカベースの磁気ビーズの仕組み 220012 フラグメントのソートを改善する

導入

分子生物学の研究では, the sorting and purification of DNA fragments is a key link to obtain high-quality sequencing data. Traditional methods often face problems such as cumbersome operation, low efficiency and unstable recovery rate. の 220012 シリカベースの磁気ビーズ introduced by Shanghai Lingjun Biotechnology Co., 株式会社. provide an efficient and reliable solution to this problem.

1. Small size, high performance

の 220012 magnetic beads were designed with the high standards of fragment sorting in mind. Its average particle size of 400 nm gives the beads a large specific surface area, which significantly increases the binding capacity of small nucleic acid fragments.

The modified silicone hydroxyl group (Si-OH) on the surface of the beads enables efficient and specific adsorption of nucleic acids, ensuring precise capture of target fragments in complex sample matrices.

The monodisperse nature of the beads ensures homogeneity in the suspension, resulting in excellent reproducibility of the results of each sorting operation, which lays a solid foundation for the stability of the experimental data.

2.Experimental investigation, results demonstration

A certain concentration of binding solution containing NaCl and PEG was prepared and mixed with 220012 magnetic beads to form a magnetic bead suspension, 100 μl of sample (containing a total amount of 1 μg of dye-free DNA Marker with a size of 100-5,000 血圧) was added, and the first round was added with 2×-0.45× volume of sorting magnetic beads for DNA fragment purification, それぞれ. In the second round, 0.15-0.2× volume of sorting magnetic beads were added, and the sorted products were detected by electrophoresis using a 1.2% agarose gel.

The unilateral recovery of DNA fragments is shown in the table below:

ビーズ:DNA	≥1.2×	1-0.8×	0.7-0.6×	0.58-0.5×	0.5×	0.45×
DNA sorting	≥100bp	≥200bp	≥300bp	≥400bp	≥500bp	＞1000bp

Bilateral recovery of DNA fragments is shown in the table below:

First Volume Ratio （Beads:DNA）	0.8×	0.7×	0.6×	0.58×	0.5×	0.45×
Second Volume Ratio （Beads:DNA）	0.2×	0.2×	0.2×	0.15×	0.15×	0.15×
DNA sorting	200-400血圧	300-500血圧	300-600血圧	300-700血圧	300-1000血圧	400-2000血圧

3. Precise sorting, wide range of applications

The core application of 220012 magnetic beads is the precise sorting and purification of DNA fragments. It can efficiently purify DNA fragments in the recovery range of 100 bp to 50 kb.

In the field of NGS sequencing, the beads have excellent performance and are suitable for PCR product purification, sequencing reaction product purification and the key DNA fragment sorting step, which can effectively remove primer dimers and other interfering substances to improve the quality of library construction.

Especially in the research of circulating free DNA (cfDNA) and circulating tumour DNA (ctDNA), 例えば, in the fields of prenatal diagnosis and early tumour screening, の 220012 magnetic beads can effectively enrich trace amounts of target nucleic acids in serum/plasma and provide high-purity templates for downstream analysis.

4. Simple handling, multiplying efficiency

Compared with the traditional agarose gel recovery method, 220012 magnetic beads can be operated without centrifugation, electrophoresis or exposure to toxic reagents, which greatly simplifies the experimental steps.

The excellent suspension properties (half-settling time > 1 時間) make the beads easy to mix and manipulate in solution, reducing experimental errors caused by uneven settling.

The beads have good magnetic response (magnetic saturation strength ≥50 emu/g), can be quickly separated under the action of the magnetic field, shorten the waiting time for experiments, and easily adapted to automated liquid workstations to achieve high-throughput sample processing, which significantly improves the efficiency of laboratory work.

5. 安定した, reliable and trustworthy

As a high-tech enterprise that has passed many international quality management system certifications, Shanghai Lingjun Biotechnology produces 220012 magnetic beads with small batch-to-batch variation and stable quality.

Strict quality control standards ensure that the performance of each batch of magnetic beads is highly consistent, so that the data of experimental projects carried out at different times are comparable, providing a guarantee for the continuity and reliability of scientific research work.

The magnetic beads are available in a variety of packaging sizes (from 2mL to 1000mL), which can meet the needs of both small-scale trials in scientific research and large-scale industrial production.

サプライヤー

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光, その他の技術分野.

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