Lnjnbio magnetiske perler til polysaccharider og polyfenoler Plante-DNA-ekstraktionssæt 160863

(Magnetisk perlemetode)

【Produktintroduktion】

For various polysaccharide and polyphenol plant samples, the nucleic acid is released under the action of lysis solution, the nucleic acid is separated from other impurities in the sample through the adsorption of magnetic beads, og skylles derefter med vaskeopløsning for at fjerne proteiner, polysaccharider, uorganiske saltioner, osv., og endelig, efter eluering, the purified nucleic acid is obtained, og the nucleic acid product obtained can be used in experiments such as PCR amplification, nukleinsyrepåvisning og analyse, molekylær hybridisering, genmutationspåvisning, og sekventering.

【Produktfordele】

1, High purity and yield of the extracted product, and for samples with high content of polysaccharides and polyphenols, it also achieves good extraction results;

2, Versatile and suitable for genomic DNA extraction from various polysaccharide and polyphenol plant samples；

3,Sikker at bruge, intet behov for formaling af flydende nitrogen, produktet indeholder ikke phenol, chloroform og andre skadelige reagenser.

【Produktbillede】

 

【Eksperimentel sag】

Case 1: Dried leaves of different polysaccharide polyphenol plants were used to extract genomic DNA using our polysaccharide polyphenol plant genomic DNA extraction reagent, and the results were as follows:

Ingen. number	Prøve	Sample intake（mg）	A260/A280	A260/A230	Konc.(ng/µL)	Yield（µg）
1	Dandelion dried leaves	5 mg	1.767	1.527	264.8	26.48
2			1.779	1.488	188.15	18.82
Gns			1.773	1.5075	226.475	22.65
3	Perilla dried leaves	5 mg	1.85	1.807	267.15	26.72
4			1.84	1.729	266.6	26.66
Gns			1.845	1.768	266.875	26.69
5	Mint dried leaves	5 mg	1.784	1.51	173.8	17.38

Peak mønster diagram:

Dandelion dried leaves:

Perilla dried leaves:

Mint dried leaves:

Elektroferogrammet er vist nedenfor:

M:Marker（2K，1K，750bp，500bp，250bp，100bp);

1-2：5mg Dandelion dried leaves

3-4：5mg Perilla dried leaves

5：5mg Mint dried leaves

Resultatanalyse:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

① The values of A260/A280 were in the range of 1.75~1.85, and the values of A260/A230 were above 1.4, and the peak shapes were complete, which proved that the extracted products were of high purity.

② The nucleic acid extraction yields of different plant leaf samples were above 15 µg.

The results of agarose gel electrophoresis showed that:

The samples of different plant leaves have bright bands and good integrity.

Som konklusion, the extracted products of different polysaccharides and polyphenols from dried plant leaves were of high purity, højt udbytte og komplette elektroforetiske bånd.

Sag 2:Fresh leaves of different polysaccharide polyphenol plants were used to extract genomic DNA using our polysaccharide polyphenol plant genomic DNA extraction reagent, and the results were as follows:

No.	Prøve	Sample intake（mg）	A260/A280	A260/A230	Konc.(ng/µL)	Yield（µg）
1	Dandelion fresh leaves	15mg	1.84	1.698	44.4	4.44
2	Perilla fresh leaves	15mg	1.745	1.379	110.1	11.01
3	Ospecios fresh leaves	10mg	1.755	1.463	55.7	5.57
4	Dichondra fresh leaves	15mg	1.838	1.661	64.65	6.47
5	Desmodium fresh leaves	15mg	1.843	1.777	89.85	8.99
6	Alternanthera philoxeroides fresh leaves	15mg	1.855	1.79	39.65	3.97
7	Plantain fresh leaves	15mg	1.872	1.969	104.2	10.42

Peak mønster diagram:

Dandelion fresh leaves:

Perilla fresh leaves

Ospecios fresh leaves

Dichondra fresh leaves

Desmodium fresh leaves

Alternanthera philoxeroides Fresh leaves

Plantain fresh leaves

Elektroferogrammet er vist nedenfor:

M:Marker（2K，1K，750bp，500bp，250bp，100bp);

1：15mg Dandelion fresh leaves

2：15mg Perilla fresh leaves

3：10mg Ospecios fresh leaves

4：15mg Dichondra fresh leaves

5：15mg Desmodium fresh leaves

6：15mg Alternanthera philoxeroides fresh leaves

7：15mg Plantain fresh leaves

Resultatanalyse:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

① For the samples of different polysaccharide and polyphenol plants, the reagent can stably extract nucleic acids with excellent yield and purity, which proves the versatility of the reagent.

② The values of A260/A280 were in the range of 1.75~1.9, and the values of A260/A230 were above 1.4, and the peak shapes were complete, which proved that the extracted products were of high purity.

③The nucleic acid extraction yield of plant leaf samples of different polysaccharide polyphenol plants was above 4 µg.

The results of agarose gel electrophoresis showed that:

①All the samples of different plant leaves have bright DNA bands with good integrity.

Som konklusion, the fresh leaf samples of different polysaccharide polyphenol plants were extracted with high purity, højt udbytte og komplette elektroforetiske bånd.

【ENanvendelsesområde】

The extracted and purified DNA of this kit can be directly used in biological experiments such as PCR, Real-time PCR, enzymfordøjelse, SNP genotyping, Next-generation sequencin, biblioteksbyggeri, molecular breeding and so on.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har relaterede behov, Du er velkommen til at kontakte os på sales01@lingjunbio.com.

Leverandørintroduktion

Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 og er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.

Vi har stor erfaring med udvinding og oprensning af nukleinsyre, proteinoprensning, celleadskillelse, kemiluminescens og andre tekniske områder.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har relaterede behov, Du er velkommen til at kontakte os på sales01@lingjunbio.com.