Lnjnbio Magnéitesch Perlen fir Polysacchariden a Polyphenole Planzen DNA Extraktioun Kit 160863

(Magnéitesch Bead Method)

【Produktintroduktioun】

For various polysaccharide and polyphenol plant samples, the nucleic acid is released under the action of lysis solution, the nucleic acid is separated from other impurities in the sample through the adsorption of magnetic beads, an dann duerch Wäschléisung gespullt fir Proteinen ze entfernen, polysaccharides, anorganesch Salzionen, etc., an endlech, no der Eluéierung, the purified nucleic acid is obtained, an the nucleic acid product obtained can be used in experiments such as PCR amplification, Nukleinsäure Detektioun an Analyse, molekulare Hybridiséierung, gene mutation detection, an sequencing.

【Produktvirdeeler】

1, High purity and yield of the extracted product, and for samples with high content of polysaccharides and polyphenols, it also achieves good extraction results;

2, Versatile and suitable for genomic DNA extraction from various polysaccharide and polyphenol plant samples；

3,Sécher ze benotzen, kee Besoin fir flësseg Stickstoff Schleifen, d'Produkt enthält keng Phenol, Chloroform an aner schiedlech Reagenz.

【Produktbild】

 

【Experimentell Fall】

Case 1: Dried leaves of different polysaccharide polyphenol plants were used to extract genomic DNA using our polysaccharide polyphenol plant genomic DNA extraction reagent, an d'Resultater waren wéi follegt:

Nee. number	Prouf	Sample intake（mg）	A260/A280	A260/A230	Konz.(ng/µL)	Yield（µg）
1	Dandelion dried leaves	5 mg	1.767	1.527	264.8	26.48
2			1.779	1.488	188.15	18.82
Avg			1.773	1.5075	226.475	22.65
3	Perilla dried leaves	5 mg	1.85	1.807	267.15	26.72
4			1.84	1.729	266.6	26.66
Avg			1.845	1.768	266.875	26.69
5	Mint dried leaves	5 mg	1.784	1.51	173.8	17.38

Peak Muster Diagramm:

Dandelion dried leaves:

Perilla dried leaves:

Mint dried leaves:

Den Elektropherogramm gëtt hei ënnen gewisen:

M:Marker (2K，1K，750bp，500bp，250bp，100bp);

1-2：5mg Dandelion dried leaves

3-4：5mg Perilla dried leaves

5：5mg Mint dried leaves

Resultat Analyse:

D'Resultater vum Ultra-Micro Nucleic Acid Assay (UMNA) Test huet dat gewisen:

① The values of A260/A280 were in the range of 1.75~1.85, an d'Wäerter vun A260 / A230 waren uewen 1.4, and the peak shapes were complete, wat bewisen huet datt déi extrahéiert Produkter vu héijer Rengheet waren.

② The nucleic acid extraction yields of different plant leaf samples were above 15 µg.

D'Resultater vun der Agarosegel Elektrophorese weisen dat:

The samples of different plant leaves have bright bands and good integrity.

Als Conclusioun, the extracted products of different polysaccharides and polyphenols from dried plant leaves were of high purity, héich nozeginn a komplett electrophoretic Bands.

Fall 2:Fresh leaves of different polysaccharide polyphenol plants were used to extract genomic DNA using our polysaccharide polyphenol plant genomic DNA extraction reagent, an d'Resultater waren wéi follegt:

No.	Prouf	Sample intake（mg）	A260/A280	A260/A230	Konz.(ng/µL)	Yield（µg）
1	Dandelion fresh leaves	15mg	1.84	1.698	44.4	4.44
2	Perilla fresh leaves	15mg	1.745	1.379	110.1	11.01
3	Ospecios fresh leaves	10mg	1.755	1.463	55.7	5.57
4	Dichondra fresh leaves	15mg	1.838	1.661	64.65	6.47
5	Desmodium fresh leaves	15mg	1.843	1.777	89.85	8.99
6	Alternanthera philoxeroides fresh leaves	15mg	1.855	1.79	39.65	3.97
7	Plantain fresh leaves	15mg	1.872	1.969	104.2	10.42

Peak Muster Diagramm:

Dandelion fresh leaves:

Perilla fresh leaves

Ospecios fresh leaves

Dichondra fresh leaves

Desmodium fresh leaves

Alternanthera philoxeroides Fresh leaves

Plantain fresh leaves

Den Elektropherogramm gëtt hei ënnen gewisen:

M:Marker (2K，1K，750bp，500bp，250bp，100bp);

1：15mg Dandelion fresh leaves

2：15mg Perilla fresh leaves

3：10mg Ospecios fresh leaves

4：15mg Dichondra fresh leaves

5：15mg Desmodium fresh leaves

6：15mg Alternanthera philoxeroides fresh leaves

7：15mg Plantain fresh leaves

Resultat Analyse:

D'Resultater vum Ultra-Micro Nucleic Acid Assay (UMNA) Test huet dat gewisen:

① For the samples of different polysaccharide and polyphenol plants, the reagent can stably extract nucleic acids with excellent yield and purity, which proves the versatility of the reagent.

② The values of A260/A280 were in the range of 1.75~1.9, an d'Wäerter vun A260 / A230 waren uewen 1.4, and the peak shapes were complete, wat bewisen huet datt déi extrahéiert Produkter vu héijer Rengheet waren.

③The nucleic acid extraction yield of plant leaf samples of different polysaccharide polyphenol plants was above 4 µg.

D'Resultater vun der Agarosegel Elektrophorese weisen dat:

①All the samples of different plant leaves have bright DNA bands with good integrity.

Als Conclusioun, the fresh leaf samples of different polysaccharide polyphenol plants were extracted with high purity, héich nozeginn a komplett electrophoretic Bands.

【AUwendungsberäich】

The extracted and purified DNA of this kit can be directly used in biological experiments such as PCR, Real-time PCR, Enzym Verdauung, SNP genotyping, Next-generation sequencin, Bibliothéik Bau, molecular breeding and so on.

Eis Produkter gi wäit a ville Beräicher benotzt, wéi medizinesch Tester, genetesch Tester, Universitéit Fuerschung, genetesch Zucht, an esou weider. Mir liwweren net nëmmen Produkter awer kënnen och OEM maachen, ODM, an aner Besoinen. Wann Dir verbonne Besoinen hutt, w.e.g. kontaktéiert eis op sales01@lingjunbio.com.

Fournisseur Aféierung

Shanghai Lingjun Biotechnology Co., Ltd., Ltd.gouf etabléiert an 2016 an ass e professionnelle Hiersteller vu biomagnetesche Materialien an Nukleinsäure Extraktiounsreagenz.

Mir hu räich Erfahrung an Nukleinsäure Extraktioun an Offäll, Protein Reinigung, Zell Trennung, Chemilumineszenz an aner technesch Felder.

Eis Produkter gi wäit a ville Beräicher benotzt, wéi medizinesch Tester, genetesch Tester, Universitéit Fuerschung, genetesch Zucht, an esou weider. Mir liwweren net nëmmen Produkter awer kënnen och OEM maachen, ODM, an aner Besoinen. Wann Dir verbonne Besoinen hutt, w.e.g. kontaktéiert eis op sales01@lingjunbio.com.