Extraction of genomic DNA from whea(magnetic bead method）

Wheat is one of the major food crops in China, so research on its molecular biology is also very important and the technology is becoming increasingly advanced. Whether it is gene cloning and transformation, genetic map construction, specific genotype detection, molecular marker-assisted breeding or DNA fingerprint database construction, they are widely applied in wheat breeding. High-quality DNA extraction is the basis for systematic analysis and research on plants at the molecular level. There are many methods for extracting plant genomic DNA, but traditional methods use harmful reagents such as phenol and chloroform, and involve many steps and take a long time. Therefore, it is recommended to use the simple, rapid, non-toxic and environmentally friendly magnetic bead method for extracting plant genomic DNA.

The plant genomic DNA extraction reagent (magnetic bead method) of Shanghai Lingjun Biotechnology Co., Ltd. is mainly used for extracting genomic DNA from various tissues of different plants. The lysis buffer directly lyses the plant samples to release nucleic acids. Under the action of the binding buffer, the magnetic beads selectively adsorb the nucleic acids in the lysis buffer. The washing buffer is used to clean the magnetic beads to remove a small amount of impurities adsorbed on them. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acids, thus obtaining high-quality genomic DNA. The entire process is convenient to operate and is especially suitable for automated nucleic acid extraction platforms. The extracted genomic DNA has a high yield, high purity, stable and reliable quality, and can be applied to various downstream molecular biology experiments.

The product advantages of our company’s plant genomic DNA extraction reagent (magnetic bead method) are as follows:

1. It can achieve excellent extraction results for both fresh and dried samples.

2. The extracted products have high purity, high yield, and complete electrophoretic bands.

3. It has strong universality and is suitable for extracting genomic DNA from samples such as leaves, fruits, and seeds of different types of plants.

4. The usage process is safe and does not require liquid nitrogen grinding. The product does not contain harmful reagents such as phenol and chloroform.

5. This kit can be used for manual extraction or can be well adapted for various high-throughput extraction instruments.

6. It is fast and simple. The entire extraction process can yield high-purity genomes within 20-60 minutes.

We used wheat leaves as the experimental material and extracted the wheat genomic DNA using the plant genomic DNA extraction reagent (magnetic bead method) provided by Shanghai Lingjun Biotechnology Co., Ltd. The reagent kit included lysis binding solution, magnetic beads, PK, DTT, washing solution, and elution solution, among other reagents.

 The experimental results are as follows:

Number	Sample	Sampling Amount	A260/A280	A260/A230	Conc.(ng/ul)	Yield（μg）
1	wheat leaf	20mg	1.881	2.223	372.3	37.2
2	wheat leaf	20mg	1.879	2.216	375.35	37.5
3	wheat leaf	20mg	1.871	2.212	371	37.1
4	wheat leaf	20mg	1.878	2.21	370.95	37.1

Electrophoresis results:

M:Marker（2K，1K，750bp，500bp，250bp，100bp）;

1-4：wheat leaf（20mg）

Result Analysis:

The test results of the ultra-micro nucleic acid analyzer show:

① The value of A260/A280 is above 1.8, and the value of A260/A230 is above 2.0, indicating that the purity of the extracted products is high.

② The nucleic acid extraction yield of 20mg wheat leaf samples is above 37 µg.

The results of agarose gel electrophoresis show:

① Wheat leaves all have bright bands and good integrity.

In conclusion: Using this kit to extract the genomic DNA of wheat leaf samples, the purity, yield and electrophoretic bands of the extracted products are all high.

Supplier

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 which is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence, and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but also can undertake OEM, ODM, and other needs. If you have a related need, please feel free to contact us at sales01@lingjunbio.com.