LnjnBio 磁気ビーズの核酸抽出への応用

1.導入

LnjnBio magnetic beads which include シリカベースの磁気ビーズ そして Carboxyl-based magnetic beads are high-performance nanomaterials developed based on magnetic separation technology. They serve as a core tool in modern nucleic acid (DNA/RNA) extraction and purification processes. Through sophisticated design, these beads enable efficient, 急速な, and automated capture and purification of nucleic acids, making them widely applicable in life sciences, 分子診断, 法医学, その他の分野.

2. Basic Composition and Structure

2.1 Core Material:

Superparamagnetic nanoparticles ( iron oxide, Fe₃O₄). These exhibit magnetism under an external magnetic field but retain no magnetic residue once the field is removed.

2.2 Surface Coating:

2.2.1 シリカベースの磁気ビーズ – シラノール基 (SiO₂): Bind nucleic acids via electrostatic interactions (dependent on high-salt and low-pH conditions).

2.2.2 Carboxyl-based magnetic beads – カルボキシル基 (-コー): Covalently bind nucleic acids with the assistance of activators such as PEG or EDC.

2.2.3 Other modifications: Including amino groups and streptavidin (for capturing biotinylated nucleic acids).

3. Working Principle

Adsorption: Surface functional groups of the magnetic beads bind to nucleic acids in a buffer solution containing chaotropic salts (例えば, guanidine hydrochloride).

Separation: An external magnetic field is applied to adsorb the magnetic bead-nucleic acid complexes, and the supernatant (containing impurities) is discarded.

洗浄: Contaminants such as proteins and salts are removed using ethanol or buffer solutions.

溶出: Nucleic acids are released from the beads into a low-salt or aqueous solution, yielding purified nucleic acids.

4. Technical Advantages of LnjnBio magnetic ビーズ

Compared to traditional methods such as centrifugal column-based extraction and phenol-chloroform extraction, Lingjun magnetic beads offer significant advantages:

高い効率とスピード: The entire process can be completed in 15–30 minutes, significantly reducing experimental time and improving workflow efficiency.

High Throughput and Automation Compatibility: The simple workflow allows easy integration with automated nucleic acid extraction systems, enabling parallel processing of 96-well plates or even higher throughputs, meeting the demands of large-scale screening and research.

高純度および高収率: Lingjun beads provide high binding capacity and consistent recovery rates thanks to specialized surface coating technology and precise particle size control. The extracted nucleic acids exhibit high purity (A260/A280比) and are suitable for downstream sensitive applications.

安全性と環境への配慮: The use of toxic organic solvents such as phenol and chloroform is eliminated, reducing health risks to operators and environmental pollution.

Broad Applicability and Flexibility: An optimized magnetic bead product can be adapted to various sample types, 全血を含む, 血清, 組織, 細胞, 植物, 細菌, and viruses, offering strong versatility.

5. Key Application Scenarios of LnjnBio magnetic Beads

Life Science Research: Extraction of genomic DNA/RNA from various animals, 植物, and microorganisms; プラスミドDNA, total RNA, and miRNA extraction.

Molecular Diagnostics: Sample preparation for PCR, qPCR, and NGS.

病原体の検出: Viral RNA extraction (例えば, for COVID-19 testing).

Scientific Research: Genomics and transcriptomics studies.

Forensic Science: Trace DNA extraction.

6. Key Considerations for Selecting LnjnBio magnetic ビーズ

Bead Size: Ranging from 800 nm to 2 μm. Different sizes offer varying adsorption efficiencies and suspension properties, allowing users to select beads based on experimental requirements.

Surface Functional Groups: Silica-based and carboxyl-based beads are available. Silica-based beads are preferred for nucleic acid extraction, while carboxyl-based beads are suitable for specific requirements.

Magnetic Properties: Saturation magnetization of 60–80 emu/g ensures strong magnetic performance, meeting diverse user needs.

7. Precautions for Use

Buffer Optimization: Salt concentration and pH must be compatible with the magnetic beads.

Avoid Freezing and Centrifugation: These actions may cause irreversible damage to the nucleic acids.

Over-Drying: Excessive drying of the beads before elution may lead to irreversible aggregation, reducing elution efficiency.

サプライヤー

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光, その他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなくOEMも承ります, ODM, その他のニーズ. 関連するニーズがある場合, お気軽にお問い合わせください .