Anwendung von LnjnBio-Magnetkügelchen bei der Nukleinsäureextraktion

1.Einführung

LnjnBio magnetic beads which include Magnetische Perlen auf Silica-Basis Und Carboxyl-based magnetic beads are high-performance nanomaterials developed based on magnetic separation technology. They serve as a core tool in modern nucleic acid (DNA/RNA) extraction and purification processes. Through sophisticated design, these beads enable efficient, schnell, and automated capture and purification of nucleic acids, making them widely applicable in life sciences, Molekulare Diagnostik, forensische Wissenschaft, und anderen Bereichen.

2. Basic Composition and Structure

2.1 Kernmaterial:

Superparamagnetic nanoparticles ( iron oxide, Fe₃O₄). These exhibit magnetism under an external magnetic field but retain no magnetic residue once the field is removed.

2.2 Surface Coating:

2.2.1 Magnetische Perlen auf Silica-Basis – Silanolgruppen (SiO₂): Bind nucleic acids via electrostatic interactions (dependent on high-salt and low-pH conditions).

2.2.2 Carboxyl-based magnetic beads – Carboxylgruppen (-COOH): Covalently bind nucleic acids with the assistance of activators such as PEG or EDC.

2.2.3 Other modifications: Including amino groups and streptavidin (for capturing biotinylated nucleic acids).

3. Working Principle

Adsorption: Surface functional groups of the magnetic beads bind to nucleic acids in a buffer solution containing chaotropic salts (z.B., guanidine hydrochloride).

Separation: An external magnetic field is applied to adsorb the magnetic bead-nucleic acid complexes, und der Überstand (containing impurities) wird verworfen.

Waschen: Contaminants such as proteins and salts are removed using ethanol or buffer solutions.

Elution: Nucleic acids are released from the beads into a low-salt or aqueous solution, yielding purified nucleic acids.

4. Technical Advantages of LnjnBio magnetic Perlen

Compared to traditional methods such as centrifugal column-based extraction and phenol-chloroform extraction, Lingjun magnetic beads offer significant advantages:

Hohe Effizienz und Geschwindigkeit: The entire process can be completed in 15–30 minutes, significantly reducing experimental time and improving workflow efficiency.

High Throughput and Automation Compatibility: The simple workflow allows easy integration with automated nucleic acid extraction systems, enabling parallel processing of 96-well plates or even higher throughputs, meeting the demands of large-scale screening and research.

Hohe Reinheit und Ausbeute: Lingjun beads provide high binding capacity and consistent recovery rates thanks to specialized surface coating technology and precise particle size control. The extracted nucleic acids exhibit high purity (A260/A280-Verhältnis) and are suitable for downstream sensitive applications.

Safety and Environmental Friendliness: The use of toxic organic solvents such as phenol and chloroform is eliminated, reducing health risks to operators and environmental pollution.

Breite Anwendbarkeit und Flexibilität: An optimized magnetic bead product can be adapted to various sample types, including whole blood, Serum, Gewebe, Zellen, Pflanzen, Bakterien, and viruses, offering strong versatility.

5. Key Application Scenarios of LnjnBio magnetic Beads

Life Science Research: Extraction of genomic DNA/RNA from various animals, Pflanzen, and microorganisms; Plasmid-DNA, total RNA, and miRNA extraction.

Molekulare Diagnostik: Sample preparation for PCR, qPCR, and NGS.

Erkennung von Krankheitserregern: Viral RNA extraction (z.B., for COVID-19 testing).

Scientific Research: Genomics and transcriptomics studies.

Forensische Wissenschaft: Trace DNA extraction.

6. Key Considerations for Selecting LnjnBio magnetic Perlen

Bead Size: Ranging from 800 nm to 2 μm. Different sizes offer varying adsorption efficiencies and suspension properties, allowing users to select beads based on experimental requirements.

Surface Functional Groups: Silica-based and carboxyl-based beads are available. Silica-based beads are preferred for nucleic acid extraction, while carboxyl-based beads are suitable for specific requirements.

Magnetic Properties: Saturation magnetization of 60–80 emu/g ensures strong magnetic performance, meeting diverse user needs.

7. Precautions for Use

Buffer Optimization: Salt concentration and pH must be compatible with the magnetic beads.

Avoid Freezing and Centrifugation: These actions may cause irreversible damage to the nucleic acids.

Over-Drying: Excessive drying of the beads before elution may lead to irreversible aggregation, reducing elution efficiency.

Anbieter

Shanghai Lingjun Biotechnology Co., Ltd.wurde gegründet in 2016 Das ist ein professioneller Hersteller von biomagnetischen Materialien und Nukleinsäure-Extraktionsreagenzien.

Wir verfügen über umfangreiche Erfahrung in der Extraktion und Reinigung von Nukleinsäuren, Proteinreinigung, Zelltrennung, Chemilumineszenz, und anderen technischen Bereichen.

Unsere Produkte werden in vielen Bereichen weit verbreitet eingesetzt, wie zum Beispiel medizinische Tests, Gentests, universitäre Forschung, genetische Zucht, und so weiter. Wir liefern nicht nur Produkte, sondern können auch OEM übernehmen, ODM, und andere Bedürfnisse. Wenn Sie einen entsprechenden Bedarf haben, Nehmen Sie gerne Kontakt zu uns auf .