Anvendelse av LnjnBio magnetiske perler i nukleinsyreekstraksjon

1.Introduksjon

LnjnBio magnetic beads which include Silikabaserte magnetiske perler og Carboxyl-based magnetic beads are high-performance nanomaterials developed based on magnetic separation technology. They serve as a core tool in modern nucleic acid (DNA/RNA) extraction and purification processes. Through sophisticated design, these beads enable efficient, rask, and automated capture and purification of nucleic acids, making them widely applicable in life sciences, molekylær diagnostikk, rettsmedisinsk vitenskap, og andre felt.

2. Basic Composition and Structure

2.1 Core Material:

Superparamagnetic nanoparticles ( iron oxide, Fe3O4). These exhibit magnetism under an external magnetic field but retain no magnetic residue once the field is removed.

2.2 Surface Coating:

2.2.1 Silikabaserte magnetiske perler – Silanolgrupper (SiO2): Bind nucleic acids via electrostatic interactions (dependent on high-salt and low-pH conditions).

2.2.2 Carboxyl-based magnetic beads – Karboksylgrupper (-COOH): Covalently bind nucleic acids with the assistance of activators such as PEG or EDC.

2.2.3 Other modifications: Including amino groups and streptavidin (for capturing biotinylated nucleic acids).

3. Working Principle

Adsorption: Surface functional groups of the magnetic beads bind to nucleic acids in a buffer solution containing chaotropic salts (f.eks., guanidine hydrochloride).

Atskillelse: An external magnetic field is applied to adsorb the magnetic bead-nucleic acid complexes, and the supernatant (containing impurities) is discarded.

Vasking: Contaminants such as proteins and salts are removed using ethanol or buffer solutions.

Eluering: Nucleic acids are released from the beads into a low-salt or aqueous solution, yielding purified nucleic acids.

4. Technical Advantages of LnjnBio magnetic Perler

Compared to traditional methods such as centrifugal column-based extraction and phenol-chloroform extraction, Lingjun magnetic beads offer significant advantages:

High Efficiency and Speed: The entire process can be completed in 15–30 minutes, significantly reducing experimental time and improving workflow efficiency.

High Throughput and Automation Compatibility: The simple workflow allows easy integration with automated nucleic acid extraction systems, enabling parallel processing of 96-well plates or even higher throughputs, meeting the demands of large-scale screening and research.

Høy renhet og utbytte: Lingjun beads provide high binding capacity and consistent recovery rates thanks to specialized surface coating technology and precise particle size control. The extracted nucleic acids exhibit high purity (A260/A280-forhold) and are suitable for downstream sensitive applications.

Safety and Environmental Friendliness: The use of toxic organic solvents such as phenol and chloroform is eliminated, reducing health risks to operators and environmental pollution.

Bred anvendelighet og fleksibilitet: An optimized magnetic bead product can be adapted to various sample types, inkludert fullblod, serum, vev, celler, planter, bakterie, and viruses, offering strong versatility.

5. Key Application Scenarios of LnjnBio magnetic Beads

Life Science Research: Extraction of genomic DNA/RNA from various animals, planter, and microorganisms; plasmid DNA, total RNA, and miRNA extraction.

Molekylær diagnostikk: Sample preparation for PCR, qPCR, and NGS.

Pathogen Detection: Viral RNA extraction (f.eks., for COVID-19 testing).

Scientific Research: Genomics and transcriptomics studies.

Forensic Science: Trace DNA extraction.

6. Key Considerations for Selecting LnjnBio magnetic Perler

Bead Size: Ranging from 800 nm to 2 μm. Different sizes offer varying adsorption efficiencies and suspension properties, allowing users to select beads based on experimental requirements.

Surface Functional Groups: Silica-based and carboxyl-based beads are available. Silica-based beads are preferred for nucleic acid extraction, while carboxyl-based beads are suitable for specific requirements.

Magnetic Properties: Saturation magnetization of 60–80 emu/g ensures strong magnetic performance, meeting diverse user needs.

7. Precautions for Use

Buffer Optimization: Salt concentration and pH must be compatible with the magnetic beads.

Avoid Freezing and Centrifugation: These actions may cause irreversible damage to the nucleic acids.

Over-Drying: Excessive drying of the beads before elution may lead to irreversible aggregation, reducing elution efficiency.

Leverandør

Shanghai Lingjun Biotechnology Co., Ltd.ble etablert i 2016 som er en profesjonell produsent av biomagnetiske materialer og nukleinsyreekstraksjonsreagenser.

Vi har rik erfaring innen utvinning og rensing av nukleinsyre, proteinrensing, celleseparasjon, kjemiluminescens, og andre tekniske felt.

Våre produkter er mye brukt på mange felt, som medisinsk testing, genetisk testing, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke bare produkter, men kan også påta oss OEM, ODM, og andre behov. Hvis du har et relatert behov, ta gjerne kontakt med oss .