Een-Schrëtt Lysis a Bindung: Lingjun Bio Whole Blood Genomic DNA Extraktioun Kit

With the rapid advancement of NGS (next-generation sequencing) and liquid biopsy, the efficiency and quality of genomic DNA extraction from blood samples directly impact the entire workflow, from library preparation to variant detection. As cutting-edge applications such as early-stage cancer screening and MRD monitoring place ever-higher demands on testing throughput and sensitivity, traditional multi-step, batch-based DNA extraction protocols are no longer sufficient to meet the requirements of precision medicine for speed, stability and accuracy.

Shanghai Lingjun Biotechnology Co., Ltd., Ltd.’s Whole Blood Genomic DNA Extraction Kit (Magnéitesch Bead Method) has redefined the standards of efficiency in genomic DNA extraction from blood through its revolutionary one-step lysis-binding design. In traditional procedures, sample lysis and nucleic acid binding are typically carried out in two separate steps. This not only takes a considerable amount of time but also requires lysis to be performed outside the instrument or an intermediate pause to add alcohol, making the process cumbersome and highly susceptible to cross-contamination. In contrast, Lingjun Biotechnology’s kit utilises a unique buffer system that enables guanidinium salts to efficiently lyse cells whilst simultaneously providing an ideal environment for magnetic beads to selectively bind nucleic acids. Lysis and binding are completed simultaneously—with a lysis time of approximately 6 Minutten, requiring no pauses or intermediate liquid additions.

When used with a fully automated magnetic bead-based nucleic acid extraction platform, the entire process—from sample loading to completion of extraction—takes just 19 Minutten, yielding high-purity genomic DNA (with an OD260/280 ratio stable between 1.8 an 1.9, and an OD260/230 ratio of ≥1.7). The extracted product exhibits intact fragments and can be used directly for PCR amplification, library construction and high-throughput sequencing. This design significantly shortens the experimental cycle, which is particularly crucial in applications requiring rapid results, such as NGS blood testing.

For the experiment, 200 μL of frozen porcine whole blood was sampled using the magnetic bead-based whole blood genomic DNA extraction kit (231006) from Shanghai Lingjun Biotechnology Co., Ltd., and a LJbio24H nucleic acid extraction instrument was employed.

As can be seen, the DNA yield is above 4 μg, the A260/280 ratio is above 1.8, the A260/230 ratio is above 1.7, and the main band is clearly visible; the sample is therefore suitable for downstream qPCR and NGS sequencing applications.

In the practical application of NGS blood testing, Shanghai Lingjun Biotechnology’s whole-blood genomic DNA extraction kit has demonstrated exceptional suitability. It reliably extracts high-purity, high-yield genomic DNA from samples ranging from 200 μL to 400 μL, meeting the stringent quality requirements for starting nucleic acids in NGS library construction. The central role of magnetic bead technology in NGS targeted sequencing, pathogen detection and precision oncology is becoming increasingly prominent—high-quality nucleic acid starting materials are the cornerstone for ensuring the accuracy of NGS data and the sensitivity of variant detection.

From core technology to practical application, Lingjun Bio’s whole-blood genomic DNA extraction kit delivers ‘faster, simpler and more reliable’ performance, providing robust support for every stage of NGS blood testing—from sample collection to results. In the race towards precision medicine, choosing the right tools is essential to staying ahead at every step.

Fournisseur

Shanghai Lingjun Biotechnology Co., Ltd., Ltd.gouf etabléiert an 2016 deen e professionnelle Hiersteller vu biomagnetesche Materialien an Nukleinsäure Extraktiounsreagenz ass.

Mir hu räich Erfahrung an Nukleinsäure Extraktioun an Offäll, Protein Reinigung, Zell Trennung, Chemilumineszenz, an aner technesch Beräicher.

Eis Produkter gi wäit a ville Beräicher benotzt, wéi medizinesch Tester, genetesch Tester, Universitéit Fuerschung, genetesch Zucht, an esou weider. Mir bidden net nëmmen Produkter, awer och kënnen OEM ënnerhuelen, ODM, an aner Besoinen. Wann Dir e verbonne Besoin hutt, weg fillen gratis eis ze kontaktéieren .