Зачем использовать экстракцию ДНК магнитными шариками при лихорадке денге?

Введение

Dengue fever, как вирусное заболевание, передающееся комарами, которое угрожает глобальному общественному здравоохранению, вспышки часто бывают быстрыми и тяжелыми.. При таких внезапных инфекционных заболеваниях, стремительный, accurate and early laboratory diagnosis is a key link to effectively control the outbreak and guide clinical treatment.

Viral nucleic acid testing (например, ОТ-ПЦР) is the “золотой стандарт” for diagnosing dengue fever. В этом процессе, nucleic acid extraction as the first step of the test, its quality and efficiency directly determine the reliability of the final results. Magnetic bead nucleic acid extraction kits, with their excellent performance, have shown irreplaceable advantages in the detection of dengue virus.

1. Преимущество

• After dengue virus infection, the viral nucleic acid (РНК) content of the patient’s blood peaks during the viraemia period, but a large amount of host DNA, белки, lipids and PCR inhibitors are also present in the sample.
• The traditional phenol-chloroform extraction method is cumbersome and time-consuming, and is prone to inhibit the subsequent PCR reaction due to the residual organic solvent.
• The magnetic bead method of nucleic acid extraction cleverly circumvents these problems. The core principle is to use surface-modified superparamagnetic nano-magnetic beads to specifically adsorb nucleic acids in a high-salt, low-pH environment, and elute them in a low-salt, high-pH environment.
• Этот “адсорбция-промывка-элюирование” process is like a tailor-made “precision adsorption and purification pipeline” for viral RNA. It can efficiently capture minute amounts of viral RNA from complex patient serum or plasma samples, and remove various impurities through strict washing steps to obtain high-purity nucleic acids. This lays a solid foundation for downstream highly sensitive RT-PCR assays and greatly reduces the risk of false-negative results.

2. Продукт

The main advantages of Shanghai LnjnBio 160013 silica-based magnetic beads in dengue virus nucleic acid extraction are reflected in their high adsorption and recovery rates, excellent suspension and stability, highly efficient magnetic response performance, and safe and environmentally friendly automation compatibility.

Strengths	Сoncrete Эxpression	Value of application
Efficiency and sensitivity	High adsorption and recovery rate:больше, чем 95%, especially good at capturing nucleic acids from micro or trace samples.	Reducing false-negative results and improving the sensitivity of the assay.
Ease of operation and stability	Excellent Suspension and Stable process	Simplicity and uniformity, reliability and comparability of test results, especially for large-scale community screening.
Purification effect and anti-interference ability	High purity extraction: it can effectively remove impurities such as proteins and polysaccharides.Wide range of adsorption: it can adsorb a wide range of nucleic acids including small fragments of DNA, РНК, viral nucleic acids.	Ensure that high quality dengue virus RNA is extracted from complex samples, например, кровь, to provide high purity templates for subsequent RT-PCR assays and ensure assay accuracy.
Automation and safety and environmental protection	Easy and safe to operate: Suitable for automatic nucleic acid extractor and fully automated workstation to achieve one-button automated extraction; the process does not require the use of toxic reagents such as phenol/chloroform.	Meet the demand for large-scale, high-throughput rapid testing during dengue outbreaks; enhance laboratory biosafety and reduce environmental pollution.

In addition to the core benefits listed in the table, there are a few technical details that are worth noting: the average particle size of this silicon-based magnetic bead is 1.2µm, and this small size provides a large specific surface area, which facilitates full contact with nucleic acids for efficient adsorption. В то же время, the magnetic saturation strength of 65±5 emu/g means that the beads can achieve rapid magnetic response and aggregation under an applied magnetic field, shortening the waiting time during operation, and can be firmly adsorbed during the removal of washing solution, avoiding loss of beads and thus ensuring the yield of nucleic acids.

3.Расширение

During the peak of dengue fever outbreaks, laboratories are often under pressure to detect large numbers of samples. Another major advantage of the magnetic bead method is its ease of automation and high-throughput operation. The entire extraction process does not require complex centrifugation and aspiration steps, just add the samples, lysate, magnetic beads and other reagents into the deep-well plate, and then all subsequent operations can be completed by the automated nucleic acid extractor. A single device can handle dozens or even hundreds of samples at a time, shortening the manual operation that originally took several hours to about half an hour.

This automated process of “sample in, result out” not only greatly liberates manpower, improves testing efficiency, and strives for a valuable “window period” for epidemic prevention and control, but also reduces human error to the maximum extent through standardised operating procedures, ensuring the stability and comparability of test results. It also ensures the stability and comparability of test results through standardised operation procedures, which is crucial for large-scale epidemiological investigation and sentinel surveillance.

Dengue patient samples are potentially infectious substances and it is crucial to ensure the biosafety of the testing personnel. The magnetic bead method is a closed-tube operation, and the whole extraction process is carried out in a sealed deep-well plate, which effectively avoids the generation of aerosols and sample leakage, and significantly reduces the risk of laboratory-acquired infections. В то же время, the method does not use toxic and volatile organic reagents such as phenol and chloroform, which reduces the health hazards to operators and pollution to the environment, and is more environmentally friendly and safe.

4.Кроме того

The magnetic bead method kits are usually provided in the form of pre-dispensed, ready-to-use kits, with stable performance and long shelf life, which are easy to be stockpiled and transported in CDCs at all levels, больницы, and laboratories in remote areas, and ensures that the emergency detection capability can be activated rapidly in the event of an epidemic outbreak.

5.summary

 Magnetic bead nucleic acid extraction technology perfectly meets the urgent demand of “быстрый, accurate and stable” for dengue virus detection by its high efficiency and high purity, convenient automation and high throughput, as well as excellent biosafety and stability. It is not only an upgrade of laboratory technology, but also an important technical pillar for building a modern and efficient infectious disease monitoring and early warning system. In the context of globalisation and in the face of the continuous threat of dengue fever and other mosquito-borne infectious diseases, the promotion and application of advanced nucleic acid extraction technologies, such as the magnetic bead method, is of far-reaching strategic significance for enhancing the public health emergency response capability and safeguarding human health.

Поставщик

Шанхайская биотехнологическая компания Линцзюнь., ОООбыл создан в 2016 который является профессиональным производителем биомагнитных материалов и реагентов для экстракции нуклеиновых кислот..

Мы имеем богатый опыт в экстракции и очистке нуклеиновых кислот., очистка белка, разделение клеток, хемилюминесценция, и другие технические области.

Наша продукция широко используется во многих областях., например, медицинское тестирование, генетическое тестирование, университетские исследования, генетическое разведение, и так далее. Мы не только поставляем продукцию, но и можем взять на себя OEM, ОДМ, и другие потребности. Если у вас есть соответствующая потребность, пожалуйста, не стесняйтесь обращаться к нам .