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Application of polystyrene microspheres in digital PCR
As the third generation of PCR, digital PCR has the advantages of absolute quantification, low sample requirement, high sensitivity, and tolerance to inhibitors, and is widely used in the detection of gene…
Nucleic Acid Extraction by Magnetic Bead Method:Revealing the Components and Functions of Reagents
The components and functions of various reagents for extracting DNA by the magnetic bead method are as follows. 1. Lysis Buffer (1) SDS (Sodium Dodecyl Sulfate): An anionic surfactant that disrupts cell membrane structures, releasing cellular contents. It can break…
Research and Advances in DNA Fragment Sorting
Solid phase reversible immobilization (SPRI) techniques have been used for DNA isolation for the past 20 years. In it, superparamagnetic carboxylated magnetic beads are used to selectively precipitate DNA molecules of different…
Why Is pH Adjustment Necessary in DNA Extraction?
pH is very important in DNA Extraction and the reasons are as follows: 1. Maintaining DNA Stability DNA remains relatively stable in solutions with a pH greater than 5 and less than 9. When the…
How Do Bio-Magnetic Beads Bind to DNA?
The technology of bio-magnetic beads binding to DNA is an indispensable part of modern molecular biology and biotechnology, providing scientists with an efficient and convenient method for separating, purifying, and enriching specific DNA sequences.…
Digital PCR Technology:Principles, Operations, and Applications
1. Experimental Principle of Digital PCR Digital PCR (dPCR) is an advanced nucleic acid molecule absolute quantification technique. Its core lies in dispersing the sample into a large number of independent micro-reaction units for PCR…
Basic strategies and application for DNA fragment sorting
The principle of magnetic bead purification of nucleic acids is based on Solid-Phase Reversible Immobilization technology（SPRI）, of which the mechanism of action is not yet very clear. Salt bridge theory is currently…
Ensuring the quality of vermicelli-easily detect cassava genes with magnetic bead method
New Year’s shopping becomes the top priority for every household as the New Year comes. Vermicelli is undoubtedly an indispensable dish on the Spring Festival table among the myriad of traditional delicacies. However, how to ensure the…
The common detection methods for endotoxin
The following methods are commonly used to detect endotoxin content in plasmids: 1, Horseshoe crab reagent color development method: This is a detection method based on the horseshoe crab reagent (Lyophilized Amebocyte…
The importance of PCR product purification
PCR stands for Polymerase Chain Reaction, a molecular biology technique used to amplify specific DNA fragments. PCR products generally cannot be used directly for downstream experiments and need to be purified first…
How to Distinguish Plasmid DNA from Chromosomal DNA During Extraction?
Plasmid DNA extraction is a fundamental and important technique in molecular biology research. The key to distinguishing plasmid DNA from chromosomal DNA lies in their differences in physical and chemical properties. Here are…
Basic strategies and methods for de-endotoxinization during plasmid DNA purification
The use of endotoxin-free plasmids should be considered when the extracted plasmid DNA is used for transfection of primary, suspension, or sensitive cells, gene silencing studies, cell injection micromanipulation, gene therapy or…