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Basic strategies and application for DNA fragment sorting
The principle of magnetic bead purification of nucleic acids is based on Solid-Phase Reversible Immobilization technology（SPRI）, of which the mechanism of action is not yet very clear. Salt bridge theory is currently…
Ensuring the quality of vermicelli-easily detect cassava genes with magnetic bead method
New Year’s shopping becomes the top priority for every household as the New Year comes. Vermicelli is undoubtedly an indispensable dish on the Spring Festival table among the myriad of traditional delicacies. However, how to ensure the…
The common detection methods for endotoxin
The following methods are commonly used to detect endotoxin content in plasmids: 1, Horseshoe crab reagent color development method: This is a detection method based on the horseshoe crab reagent (Lyophilized Amebocyte…
The importance of PCR product purification
PCR stands for Polymerase Chain Reaction, a molecular biology technique used to amplify specific DNA fragments. PCR products generally cannot be used directly for downstream experiments and need to be purified first…
How to Distinguish Plasmid DNA from Chromosomal DNA During Extraction?
Plasmid DNA extraction is a fundamental and important technique in molecular biology research. The key to distinguishing plasmid DNA from chromosomal DNA lies in their differences in physical and chemical properties. Here are…
Basic strategies and methods for de-endotoxinization during plasmid DNA purification
The use of endotoxin-free plasmids should be considered when the extracted plasmid DNA is used for transfection of primary, suspension, or sensitive cells, gene silencing studies, cell injection micromanipulation, gene therapy or…
How to efficiently extract nucleic acids from plants
In modern molecular biology research, plant nucleic acid extraction is the basis of genomics, transcriptomics, functional genomics, and other fields. However, traditional methods often face the interference of secondary metabolites such as…
How to extract DNA from bones
In scientific research or identification fields such as archaeology, forensic science, and paleontology, scientists often need to obtain genetic information about an organism from ancient bone or tooth samples. This information is…
Extracting DNA fragments of specific sizes efficiently and accurately by magnetic bead
With the rapid development of biotechnology, the isolation and purification of DNA fragments have become an indispensable part of molecular biology research. Whether for gene cloning, sequencing analysis, or other molecular biology…
The difference of units between IU/mL and copies/mL
Many small partners see the biological field always appears IU / mL and copies/mL of these two units, always confused about the difference between the two, in fact, these two units in definition, application,…
Extract RNA from water to discover the secret of liquid
Every drop of water may hide important biological information in the unknown world. With the advancement of technology, scientists have started to explore how to extract genetic material from various environments to reveal its…
What are the advantages of direct amplification magnetic beads compared to common magnetic beads?
The technology of direct amplification magnetic beads combines the technology of magnetic beads with the technology of PCR, mainly used to improve the efficiency and quality of nucleic acid extraction and can amplify nucleic acid efficiently. The…