Advantages of alcohol-free nucleic acid extraction reagent

1. Main advantages of alcohol-free extraction reagents

To avoid the risks associated with organic solvents, traditional extraction reagents rely on ethanol or isopropanol as nucleic acid stabilizers. However, these alcohols are flammable, explosive, and volatile, requiring special conditions for transportation and storage (such as low temperatures and explosion-proof packaging). In contrast, alcohol-free reagents eliminate these safety hazards through alternative formulations (such as polyethylene glycol and oil phases), reducing transportation costs and enhancing the stability of the reagent after opening.

Improving operational convenience and compatibility with automation, alcohol-containing reagents require manual intervention for addition in automated processes, whereas alcohol-free reagents can be preloaded directly into the kit, enabling fully enclosed automated operations. For example, a certain blood nucleic acid extraction kit optimizes the lysis and rinse solution formulations to complete the magnetic bead-bound nucleic acid step without pausing the instrument, reducing the extraction time to within 20 minutes.

Reducing residual interference, ethanol residues can inhibit downstream PCR and other enzymatic reactions, whereas alcohol-free reagents effectively remove impurities through washing liquid stratification techniques (such as the synergy between aqueous and oily phases), avoiding the negative impact of residues on detection sensitivity. For example, a patented technology achieves efficient nucleic acid purification by washing with PEG in one step, increasing the recovery rate by four times compared to traditional methods.

Simplifying the formula and reducing costs, traditional alcohol-free solutions often require complex components (such as proteinase K). In contrast, new reagents reduce the number of reagents and lower production costs by optimizing PEG concentration and sodium salt ratios. For example, a magnetic bead kit only requires lysis buffer, wash buffer A/B, and elution buffer, eliminating the need for additional proteinase K, significantly reducing the complexity of the kit.

To enhance environmental friendliness, alcohol-free reagents avoid the use of toxic organic solvents, in line with the trend of green chemistry.

Supplier

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 which is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence, and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but also can undertake OEM, ODM, and other needs. If you have a related need, please feel free to contact us at sales01@lingjunbio.com.