PCR Amplification Kit （General）

serves as an ideal teaching tool for fundamental experiments such as gene cloning, genetic identification, and pathogen detection.

【Product Introduction】

This 2× Taq PCR Master Mix (with Dye) contains a highly efficient thermostable DNA polymerase, dNTPs, an optimised MgCl₂ buffer system, and bromophenol blue dye. This formulation maximises operational simplicity, minimises preparation errors, ensures reproducible results and high success rates, and requires only the addition of nucleic acid dye for electrophoresis following amplification.

【Product advantages】

• User-friendly operation: Optimised premix components significantly streamline experimental procedures and lower operational barriers.
• Reliable results: Specifically optimised for teaching applications, delivering high amplification success rates, excellent reproducibility, and robust interference resistance.
• The universal version allows selection of corresponding primers for amplifying phage cDNA fragments ranging from 500bp to 1000bp, tailored to teaching experiment requirements

【Experimental Case】

An 800bp fragment designed using the 1026bp Lambda bacteriophage cDNA as template was amplified via PCR. The amplification reaction comprised a 50 μL system with 10 ng template and a primer annealing temperature of 60°C. Following amplification, 1 μL of each product was subjected to nucleic acid electrophoresis using DL 2000 as the marker under conditions of 120 V for 35 minutes. The electrophoresis results are as follows:

【Application area】

This product is suitable for teaching molecular biology experiments in life sciences, medicine, and agricultural and forestry disciplines. It can be used for fundamental experiments such as gene cloning, genetic identification, and pathogen detection, making it an ideal teaching tool.