مجموعة أدوات استخراج الحمض النووي الجينومي للأنسجة الحيوانية (طريقة الخرزة المغناطيسية, الاستخدام التعليمي فقط)

【مقدمة المنتج】

The sample releases nucleic acids under the action of the lysis buffer. Magnetic beads selectively adsorb the nucleic acids in the binding buffer, وفصلها عن الشوائب الأخرى في العينة. تبعًا, the mixture is washed with a washing buffer to remove proteins, السكريات, أيونات الملح غير العضوية, and other contaminants. أخيراً, high-quality DNA is obtained through elution.

【مزايا المنتج】

• Streamlined procedure with tailored session duration, compatible with laboratory timetables (typically 30–60 minutes)
• Optimised for educational settings, free from highly toxic organic solvents such as phenol and chloroform
• Eliminates the need for repeated centrifugation, ensuring high-purity DNA products with substantial yield and intact electrophoresis bands
• Incorporates cutting-edge magnetic bead technology to broaden students’ scientific horizons
• Suitable for extracting genomic DNA from a variety of fresh animal tissues (such as pork, chicken, جمبري, pork liver, chicken liver, إلخ.) for subsequent PCR amplification and detection.

【حالة تجريبية】

Genomic DNA extraction experiments were conducted using 5mg samples of various fresh, young tissues, yielding the following results:

【أمنطقة التطبيق 】

The nucleic acids extracted and purified using this kit may be employed in a variety of downstream molecular biology experiments, including PCR, quantitative real-time PCR, digital PCR, تقييد هضم الإنزيم, Southern blotting, التنميط الجيني SNP, تسلسل الجيل القادم, and library preparation.