مجموعة استخراج الحمض النووي الجيني للنبات (طريقة الخرزة المغناطيسية, الاستخدام التعليمي فقط)

【مقدمة المنتج】

The sample releases nucleic acids under the action of the lysis buffer. Magnetic beads selectively adsorb the nucleic acids in the binding buffer, وفصلها عن الشوائب الأخرى في العينة. تبعًا, the mixture is washed with a washing buffer to remove proteins, السكريات, أيونات الملح غير العضوية, and other contaminants. أخيراً, high-quality DNA is obtained through elution.

【مزايا المنتج】

• Streamlined procedure with flexible timing to accommodate laboratory session schedules (typically 30–60 minutes)
• Replacement of DTT with a reducing agent free from highly toxic organic solvents such as phenol and chloroform, and without pungent odours
• Eliminates the need for repeated centrifugation, ensuring high-purity, high-yield DNA products with intact electrophoresis bands
• Facilitates the cultivation of scientific thinking and standardised procedures among students, broadening their scientific horizons
• Suitable for extracting genomic DNA from the tender leaves and seeds of various plants (such as Chinese spinach, spinach, Shanghai green, Chinese cabbage, lettuce, coriander, wheat, أرز, maize, soybeans, إلخ.) for subsequent PCR amplification and detection.

【حالة تجريبية】

Genomic DNA extraction experiments were conducted using 5mg samples of various fresh, young tissues, yielding the following results:

【أمنطقة التطبيق 】

The nucleic acids extracted and purified using this kit may be employed in a variety of downstream molecular biology experiments, including PCR, quantitative real-time PCR, digital PCR, تقييد هضم الإنزيم, Southern blotting, التنميط الجيني SNP, تسلسل الجيل القادم, and library preparation.