植物ゲノムDNA抽出キット (磁気ビーズ法, 教育用途のみ)

【商品紹介】

The sample releases nucleic acids under the action of the lysis buffer. Magnetic beads selectively adsorb the nucleic acids in the binding buffer, サンプル中の他の不純物からそれらを分離する. その後, the mixture is washed with a washing buffer to remove proteins, 多糖類, 無機塩イオン, and other contaminants. ついに, high-quality DNA is obtained through elution.

【製品の利点】

• Streamlined procedure with flexible timing to accommodate laboratory session schedules (typically 30–60 minutes)
• Replacement of DTT with a reducing agent free from highly toxic organic solvents such as phenol and chloroform, and without pungent odours
• Eliminates the need for repeated centrifugation, ensuring high-purity, high-yield DNA products with intact electrophoresis bands
• Facilitates the cultivation of scientific thinking and standardised procedures among students, broadening their scientific horizons
• Suitable for extracting genomic DNA from the tender leaves and seeds of various plants (such as Chinese spinach, spinach, Shanghai green, Chinese cabbage, lettuce, coriander, wheat, 米, maize, soybeans, 等) for subsequent PCR amplification and detection.

【実験事例】

Genomic DNA extraction experiments were conducted using 5mg samples of various fresh, young tissues, yielding the following results:

【あ適用範囲】

The nucleic acids extracted and purified using this kit may be employed in a variety of downstream molecular biology experiments, including PCR, quantitative real-time PCR, デジタル PCR, 制限酵素消化, Southern blotting, SNP ジェノタイピング, 次世代シーケンス, and library preparation.