The sample releases nucleic acids under the action of the lysis buffer. Magnetic beads selectively adsorb the nucleic acids in the binding buffer, separating them from other impurities in the sample. Subsequently, the mixture is washed with a washing buffer to remove proteins, 多糖類, inorganic salt ions, and other contaminants. Finally, high-quality DNA is obtained through elution.
【Product advantages】
Streamlined procedure with tailored session duration, compatible with laboratory timetables (typically 30–60 minutes)
Optimised for educational settings, free from highly toxic organic solvents such as phenol and chloroform
Eliminates the need for repeated centrifugation, ensuring high-purity DNA products with substantial yield and intact electrophoresis bands
Incorporates cutting-edge magnetic bead technology to broaden students’ scientific horizons
Suitable for extracting genomic DNA from a variety of fresh animal tissues (such as pork, chicken, shrimp, pork liver, chicken liver, 等) for subsequent PCR amplification and detection.
【Experimental Case】
Genomic DNA extraction experiments were conducted using 5mg samples of various fresh, young tissues, yielding the following results:
No.
サンプル
A260/A280
A260/A230
Conc.(ng/μL)
1
Chicken liver
1.906
2.049
209.15
2
Pork liver
1.879
1.595
119.1
3
Fish
1.942
1.96
74.3
4
Pork
2.003
1.952
55.9
5
Chicken
1.951
2.047
197.5
6
Shrimp
1.959
1.996
166.7
【あpplication area】
The nucleic acids extracted and purified using this kit may be employed in a variety of downstream molecular biology experiments, including PCR, quantitative real-time PCR, digital PCR, restriction enzyme digestion, Southern blotting, SNP genotyping, next-generation sequencing, and library preparation.