Trealamh Asbhainte DNA Géanómaíochta Fíocháin Ainmhithe (Modh Coirnín Maighnéadach, Úsáid Oideachasúil Amháin)

【Réamhrá Táirge】

The sample releases nucleic acids under the action of the lysis buffer. Magnetic beads selectively adsorb the nucleic acids in the binding buffer, iad a scaradh ó eisíontais eile sa sampla. Ina dhiaidh sin, the mixture is washed with a washing buffer to remove proteins, polaisiúicrídí, hiain salainn neamhorgánacha, and other contaminants. Ar deireadh, high-quality DNA is obtained through elution.

【Buntáistí táirge】

• Streamlined procedure with tailored session duration, compatible with laboratory timetables (typically 30–60 minutes)
• Optimised for educational settings, free from highly toxic organic solvents such as phenol and chloroform
• Eliminates the need for repeated centrifugation, ensuring high-purity DNA products with substantial yield and intact electrophoresis bands
• Incorporates cutting-edge magnetic bead technology to broaden students’ scientific horizons
• Suitable for extracting genomic DNA from a variety of fresh animal tissues (such as pork, chicken, ribí róibéis, pork liver, chicken liver, srl.) for subsequent PCR amplification and detection.

【Experimental Case】

Genomic DNA extraction experiments were conducted using 5mg samples of various fresh, young tissues, yielding the following results:

【Alimistéar iarratais】

The nucleic acids extracted and purified using this kit may be employed in a variety of downstream molecular biology experiments, including PCR, quantitative real-time PCR, PCR digiteach, díleá einsím srian, Southern blotting, Géinitíopáil SNP, seicheamhú don chéad ghlúin eile, and library preparation.