Plant Genomic DNA Extraction Kit (Modh Coirnín Maighnéadach, Educational Use Only)

【Réamhrá Táirge】

The sample releases nucleic acids under the action of the lysis buffer. Magnetic beads selectively adsorb the nucleic acids in the binding buffer, separating them from other impurities in the sample. Subsequently, the mixture is washed with a washing buffer to remove proteins, polaisiúicrídí, inorganic salt ions, and other contaminants. Ar deireadh, high-quality DNA is obtained through elution.

【Buntáistí táirge】

• Streamlined procedure with flexible timing to accommodate laboratory session schedules (typically 30–60 minutes)
• Replacement of DTT with a reducing agent free from highly toxic organic solvents such as phenol and chloroform, and without pungent odours
• Eliminates the need for repeated centrifugation, ensuring high-purity, high-yield DNA products with intact electrophoresis bands
• Facilitates the cultivation of scientific thinking and standardised procedures among students, broadening their scientific horizons
• Suitable for extracting genomic DNA from the tender leaves and seeds of various plants (such as Chinese spinach, spinach, Shanghai green, Chinese cabbage, lettuce, coriander, wheat, rice, maize, soybeans, srl.) for subsequent PCR amplification and detection.

【Experimental Case】

Genomic DNA extraction experiments were conducted using 5mg samples of various fresh, young tissues, yielding the following results:

【Alimistéar iarratais】

The nucleic acids extracted and purified using this kit may be employed in a variety of downstream molecular biology experiments, including PCR, quantitative real-time PCR, digital PCR, restriction enzyme digestion, Southern blotting, SNP genotyping, next-generation sequencing, and library preparation.