Hvordan man sikrer kvaliteten af ​​RNA-ekstraktion fra urin og andre prøver?

Urin og skedesekret, sæd, tårer og andre prøver er blevet populære valg i forskning på grund af deres ikke-invasivitet og lette adgang. Derfor, correct pretreatment steps are crucial to extract high-quality RNA from these samples. The following are several pretreatment tips that can help improve the quality of RNA from urine and other samples.

Keeping enough sample volume

The RNA content in urine samples is relatively low, so keeping a larger sample volume is recommended, which can not only improve the recovery rate of RNA, but also increase the overall quality of RNA extraction.

Standardized sample collection

The quality of urine samples is affected by many factors, including collection time, urination frequency, osv. Establishing a standardized collection procedure can help reduce the impact of these variables on sample quality.

Use of protectants

Although urine samples are unlikely to require additional protectants when cryopreserved, in some cases, the use of protectants such as RNAlater can help maintain RNA stability.

Appropriate centrifugation

Centrifugation can help remove precipitates and other impurities in urine, ensuring that they won’t disturb the RNA extraction.

Processing of other samples

Using sperm washing solution to purify sperm can completely remove the contamination from other cells, laying a good foundation for the next step of extracting high-quality sperm RNA. For vaginal secretions, 10 mL of vaginal lavage fluid is usually collected, and the precipitate is stored in 1 mL RNAlater after centrifugation and then stored at -70℃ for later use. The best pretreatment method for tear samples is to put the tear samples into 1.5 mL RNase-free centrifuge tubes, freeze them at -70℃, and then extract RNA when needed. Low-temperature freezing can maximize the quality of RNA.

The above pretreatment strategy can improve the quality of RNA in urine and other samples effectively, providing a solid foundation for subsequent research work.

Leverandørintroduktion

Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 som er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.

Vi har stor erfaring med nukleinsyreisolering og oprensning, proteinoprensning, celleadskillelse, kemiluminescens og andre tekniske områder.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, osv. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har relaterede behov, er du velkommen til at kontakte os påsales01@lingjunbio.com.