Come garantire la qualità dell'estrazione dell'RNA dall'urina e da altri campioni？

Urina e secrezioni vaginali, sperma, lacrime e altri campioni sono diventati scelte popolari nella ricerca grazie alla loro non invasività e al facile accesso. Perciò, correct pretreatment steps are crucial to extract high-quality RNA from these samples. The following are several pretreatment tips that can help improve the quality of RNA from urine and other samples.

Keeping enough sample volume

The RNA content in urine samples is relatively low, so keeping a larger sample volume is recommended, which can not only improve the recovery rate of RNA, but also increase the overall quality of RNA extraction.

Standardized sample collection

The quality of urine samples is affected by many factors, including collection time, urination frequency, ecc. Establishing a standardized collection procedure can help reduce the impact of these variables on sample quality.

Use of protectants

Although urine samples are unlikely to require additional protectants when cryopreserved, in some cases, the use of protectants such as RNAlater can help maintain RNA stability.

Appropriate centrifugation

Centrifugation can help remove precipitates and other impurities in urine, ensuring that they won’t disturb the RNA extraction.

Processing of other samples

Using sperm washing solution to purify sperm can completely remove the contamination from other cells, laying a good foundation for the next step of extracting high-quality sperm RNA. For vaginal secretions, 10 mL of vaginal lavage fluid is usually collected, and the precipitate is stored in 1 mL RNAlater after centrifugation and then stored at -70℃ for later use. The best pretreatment method for tear samples is to put the tear samples into 1.5 mL RNase-free centrifuge tubes, freeze them at -70℃, and then extract RNA when needed. Low-temperature freezing can maximize the quality of RNA.

The above pretreatment strategy can improve the quality of RNA in urine and other samples effectively, providing a solid foundation for subsequent research work.

Introduzione del fornitore

Shanghai Lingjun Biotecnologia Co., Ltd.è stato fondato nel 2016 che è un produttore professionale di materiali biomagnetici e reagenti per l'estrazione degli acidi nucleici.

Abbiamo una ricca esperienza nell'isolamento e nella purificazione degli acidi nucleici, purificazione delle proteine, separazione cellulare, chemiluminescenza e altri campi tecnici.

I nostri prodotti sono ampiamente utilizzati in molti campi, come i test medici, test genetici, ricerca universitaria, allevamento genetico, ecc. Non solo forniamo prodotti, ma possiamo anche intraprendere l'OEM, ODM, e altre esigenze. Se hai esigenze correlate, non esitate a contattarci asales01@lingjunbio.com.