Lnjnbio Magnetic Beads for Plant DNA/RNA Extraction Kit 231011

(Magnetisk perlemetode)

【Produktintroduktion】

The Plant DNA/RNA Extraction Reagent is suitable for the fast and efficient extraction of DNA/RNA from plant tissue samples such as plant leaves, fruits, seeds, osv. The whole process is easy to operate and is especially suitable for automated nucleic acid extraction platforms. The extraction process releases nucleic acid under the action of lysis solution; magnetic beads selectively adsorb nucleic acid under the action of binding solution, separating nucleic acid from other impurities in the sample; then rinsing with washing solution to remove proteins, polysaccharider, uorganiske saltioner, osv.; og endelig, high-quality DNA/RNA is obtained by elution.The product does not require toxic reagents such as phenol, chloroform, osv., and can be applied to many types of plant tissues. It can be applied to many kinds of downstream molecular biology experiments.

【Produktfordele】

1, Easy to operate, magnetic bead method can be used with automatic nucleic acid extractor to realize automated extraction, lysis combined with one step, efficient extraction;

2, No need for repeated centrifugation to ensure the integrity of the extracted DNA/RNA;

3, Extracted products have good purity, højt udbytte og komplette elektroforetiske bånd;

4, The use of process safety, uden formaling af flydende nitrogen, produktet indeholder ikke phenol, chloroform og andre skadelige reagenser;

5, Stærk alsidighed, suitable for extracting DNA/RNA from leaves, fruits, seeds and other samples of different kinds of plants.

【Produktbillede】

 【Experimental case】

Sag 1:

 Concentration and purity values of Q5000 assay for fresh plant leaves

No.	Prøve	Sample intake(mg)	A260/A280	A260/A230	Konc.(ng/µL)
1	Baby cabbage fresh leaves	20	2.18	2.155	226.15
2		20	2.195	2.136	232.1
3		20	2.206	2.138	227
4	Hangzhou cabbage fresh leaves	20	2.184	2.137	218.65
5		20	2.191	2.108	227.3
6		20	2.239	1.936	161.55
7	Amaranth fresh leaves	20	2.16	2.05	332.85
8		20	2.169	2.072	335.35
9		20	2.165	1.963	246.2
10	Oily lettuce fresh leaves	20	2.166	1.99	222.7
11		20	2.165	1.984	230.05
12		20	2.165	1.98	237.2
13	CorianderFresh leaves	20	2.143	1.937	137.65
14		20	2.134	1.943	139.5
15		20	2.144	1.933	141.05

Elektroferogrammet er vist nedenfor:

Resultatanalyse: Fresh plant leaf sample DNA/RNA extracted with this product has good purity, højt udbytte og komplette bånd.

Case 2:Leaf samples of different plants were taken and DNA/RNA was extracted with the following results:

No.	Prøve	A260/A280	A260/A230	Konc.(ng/µL)	Udbytte(µg)
1	20mg Sweet potato leaves	1.935	2.2	772.75	77.275
2		1.934	2.206	777.65	77.765
Gns		1.9345	2.203	775.2	77.52
3	20mg Castor leaves	1.928	2.148	873.45	87.345
4		1.928	2.141	877.2	87.72
Gns		1.928	2.1445	875.325	87.5325
5	20mg Lettuce leaves	1.91	2.102	209.4	20.94
6		1.91	2.105	213.7	21.37
Gns		1.91	2.1035	211.55	21.155
7	20mg Carob tree leaves	1.899	1.846	250.4	25.04
8		1.898	1.849	245	24.5
Gns		1.8985	1.8475	247.7	24.77

Peak mønster diagram：

Sweet potato leaves：

Castor leaves：

 Lettuce leaves：

Carob tree leaves：

Total peak diagram:

Elektroferogrammet er vist nedenfor:

M:Marker（2K，1K，750bp，500bp，250bp，100bp);

1-2： 20mg Sweet potato leaves（extracted product diluted 4 times）

3-4： 20mg Castor leaves（extracted product diluted 5 times）

5-6： 20mg Lettuce leaves

7-8： 20mg Carob tree leaves

Resultatanalyse:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

①The values of A260/A280 and A260/A230 are above 1.8, and the peak shapes are complete and basically coincide, which proves that the extracted products are of high purity.

②The yields of nucleic acids extracted from different plant leaf samples were above 20 µg.

The results of agarose gel electrophoresis showed that:

①All the samples of different plant leaves have bright bands and good integrity.

Som konklusion, the extracted products of different plant leaves samples were of high purity, højt udbytte og komplette elektroforetiske bånd.

【ENpplication area】

Nucleic acids purified by this kit can be used for PCR, qPCR, Enzyme digestion, library construction and Southern-Blot.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har relaterede behov, Du er velkommen til at kontakte os på sales01@lingjunbio.com.

Leverandørintroduktion

Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 og er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.

Vi har stor erfaring med udvinding og oprensning af nukleinsyre, proteinoprensning, celleadskillelse, kemiluminescens og andre tekniske områder.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har relaterede behov, Du er velkommen til at kontakte os på sales01@lingjunbio.com.