Feabhas a chur ar an bPróiseas Réamhchóireála Samplach Fíocháin chun Cáilíocht Eastóscadh RNA a Chinntiú

Is cuid fíor-riachtanach den taighde bithleighis iad samplaí fíocháin, agus extracting high-quality RNA from tissues is crucial to the success of many experiments. The following pretreatment considerations can help you maximize the quality of RNA in tissue samples.

I. Shorten the cold ischemia time

The cold ischemia time refers to the time interval between tissue removal and storage. The shorter the time is, the lower the risk of RNA degradation. Dá bhrí sin, tissue samples should be transferred to a cold environment as quickly as possible.

II. Choose the right storage method

Although cryopreservation can maintain the integrity of RNA effectively, it may cause mechanical damage to the tissue during freezing and thawing. To mitigate this effect, protective agents such as dimethyl sulfoxide (DMSO) and glycerol can be added during the storage process to reduce the formation of ice crystals.

III. Reduce freeze-thaw cycles

Repeated freezing and thawing of tissues can lead to increased RNA degradation. Dá bhrí sin, try to minimize the number of freeze-thaw cycles of tissue samples and refrozen the remaining samples after each use.

IV. Choice of fixation method

Different fixation methods can affect the integrity of RNA in tissue samples. Mar shampla, although paraffin embedding is a commonly used fixation method, the RNA content extracted is lower and is more suitable for quantitative PCR analysis.

Following the above tissue pretreatment precautions can help researchers obtain more reliable tissue RNA samples, thereby supporting high-quality scientific research.

Réamhrá Soláthraí

Shanghai Lingjun Biteicneolaíocht Co., Teo.bunaíodh i 2016 atá ina mhonaróir gairmiúil d'ábhair bithmhaighnéadacha agus imoibrithe eastóscadh aigéid núicléacha.

Tá taithí shaibhir againn in eastóscadh agus íonú aigéad núicléasach, íonú próitéine, scaradh cille, chemiluminescence agus réimsí teicniúla eile.

Úsáidtear ár gcuid táirgí go forleathan i go leor réimsí, mar thástáil leighis, tástáil ghéiniteach, taighde ollscoile, pórú géiniteach, agus mar sin de. Ní chuirimid ar fáil ach táirgí ach freisin is féidir tabhairt faoi OEM, ODM, agus riachtanais eile. Má tá riachtanais ghaolmhara agat, bíodh leisce ort teagmháil a dhéanamh linn agdíolacháin01@lingjunbio.com.