Ekstraksi DNA genom dari whea(metode manik magnetik）

Gandum adalah salah satu tanaman pangan utama di Tiongkok, sehingga penelitian mengenai biologi molekulernya juga sangat penting dan teknologinya semakin maju. Whether it is gene cloning and transformation, genetic map construction, specific genotype detection, molecular marker-assisted breeding or DNA fingerprint database construction, they are widely applied in wheat breeding. High-quality DNA extraction is the basis for systematic analysis and research on plants at the molecular level. There are many methods for extracting plant genomic DNA, but traditional methods use harmful reagents such as phenol and chloroform, and involve many steps and take a long time. Karena itu, it is recommended to use the simple, cepat, non-toxic and environmentally friendly magnetic bead method for extracting plant genomic DNA.

The plant genomic DNA extraction reagent (metode manik magnetik) of Shanghai Lingjun Bioteknologi Co., Ltd. is mainly used for extracting genomic DNA from various tissues of different plants. The lysis buffer directly lyses the plant samples to release nucleic acids. Under the action of the binding buffer, the magnetic beads selectively adsorb the nucleic acids in the lysis buffer. The washing buffer is used to clean the magnetic beads to remove a small amount of impurities adsorbed on them. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acids, thus obtaining high-quality genomic DNA. The entire process is convenient to operate and is especially suitable for automated nucleic acid extraction platforms. The extracted genomic DNA has a high yield, kemurnian tinggi, kualitas stabil dan dapat diandalkan, and can be applied to various downstream molecular biology experiments.

The product advantages of our company’s plant genomic DNA extraction reagent (metode manik magnetik) are as follows:

1. It can achieve excellent extraction results for both fresh and dried samples.

2. The extracted products have high purity, hasil tinggi, dan pita elektroforesis lengkap.

3. It has strong universality and is suitable for extracting genomic DNA from samples such as leaves, buah-buahan, and seeds of different types of plants.

4. The usage process is safe and does not require liquid nitrogen grinding. Produk tidak mengandung reagen berbahaya seperti fenol dan kloroform.

5. This kit can be used for manual extraction or can be well adapted for various high-throughput extraction instruments.

6. It is fast and simple. The entire extraction process can yield high-purity genomes within 20-60 menit.

We used wheat leaves as the experimental material and extracted the wheat genomic DNA using the plant genomic DNA extraction reagent (metode manik magnetik) provided by Shanghai Lingjun Biotechnology Co., Ltd. The reagent kit included lysis binding solution, manik-manik magnetik, hal, DTT, washing solution, and elution solution, among other reagents.

 Hasil percobaannya adalah sebagai berikut:

Nomor	Mencicipi	Jumlah Sampel	A260/A280	A260/A230	Kesimpulan.(dari/ul)	Hasil（μg）
1	wheat leaf	20mg	1.881	2.223	372.3	37.2
2	wheat leaf	20mg	1.879	2.216	375.35	37.5
3	wheat leaf	20mg	1.871	2.212	371	37.1
4	wheat leaf	20mg	1.878	2.21	370.95	37.1

Hasil elektroforesis:

M:Penanda（2K，1K，750bp，500bp，250bp，100bp）;

1-4：wheat leaf（20mg）

Result Analysis:

The test results of the ultra-micro nucleic acid analyzer show:

① The value of A260/A280 is above 1.8, and the value of A260/A230 is above 2.0, indicating that the purity of the extracted products is high.

② The nucleic acid extraction yield of 20mg wheat leaf samples is above 37 mg.

The results of agarose gel electrophoresis show:

① Wheat leaves all have bright bands and good integrity.

Kesimpulannya: Using this kit to extract the genomic DNA of wheat leaf samples, the purity, yield and electrophoretic bands of the extracted products are all high.

Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 yang merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, kimialuminesensi, dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami .