Extração de DNA genômico do soro de leite(método de conta magnética）

O trigo é uma das principais culturas alimentares na China, portanto, a pesquisa sobre sua biologia molecular também é muito importante e a tecnologia está se tornando cada vez mais avançada. Whether it is gene cloning and transformation, genetic map construction, specific genotype detection, molecular marker-assisted breeding or DNA fingerprint database construction, they are widely applied in wheat breeding. High-quality DNA extraction is the basis for systematic analysis and research on plants at the molecular level. There are many methods for extracting plant genomic DNA, but traditional methods use harmful reagents such as phenol and chloroform, and involve many steps and take a long time. Portanto, it is recommended to use the simple, rápido, non-toxic and environmentally friendly magnetic bead method for extracting plant genomic DNA.

The plant genomic DNA extraction reagent (método de conta magnética) de Xangai Lingjun Biotecnologia Co., Ltda. is mainly used for extracting genomic DNA from various tissues of different plants. The lysis buffer directly lyses the plant samples to release nucleic acids. Under the action of the binding buffer, the magnetic beads selectively adsorb the nucleic acids in the lysis buffer. The washing buffer is used to clean the magnetic beads to remove a small amount of impurities adsorbed on them. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acids, thus obtaining high-quality genomic DNA. The entire process is convenient to operate and is especially suitable for automated nucleic acid extraction platforms. The extracted genomic DNA has a high yield, alta pureza, qualidade estável e confiável, e pode ser aplicado a vários experimentos de biologia molecular a jusante.

The product advantages of our company’s plant genomic DNA extraction reagent (método de conta magnética) are as follows:

1. It can achieve excellent extraction results for both fresh and dried samples.

2. The extracted products have high purity, alto rendimento, e bandas eletroforéticas completas.

3. It has strong universality and is suitable for extracting genomic DNA from samples such as leaves, frutas, and seeds of different types of plants.

4. The usage process is safe and does not require liquid nitrogen grinding. The product does not contain harmful reagents such as phenol and chloroform.

5. This kit can be used for manual extraction or can be well adapted for various high-throughput extraction instruments.

6. It is fast and simple. The entire extraction process can yield high-purity genomes within 20-60 minutos.

We used wheat leaves as the experimental material and extracted the wheat genomic DNA using the plant genomic DNA extraction reagent (método de conta magnética) provided by Shanghai Lingjun Biotechnology Co., Ltda. The reagent kit included lysis binding solution, contas magnéticas, PC, TDT, washing solution, and elution solution, among other reagents.

 Os resultados experimentais são os seguintes:

Número	Amostra	Quantidade de amostragem	A260/A280	A260/A230	Concentração.(de/ul)	Rendimento（μg）
1	wheat leaf	20mg	1.881	2.223	372.3	37.2
2	wheat leaf	20mg	1.879	2.216	375.35	37.5
3	wheat leaf	20mg	1.871	2.212	371	37.1
4	wheat leaf	20mg	1.878	2.21	370.95	37.1

Resultados da eletroforese:

M:Marcador（2K，1K，750bp，500bp，250bp，100bp）;

1-4：wheat leaf（20mg）

Análise de Resultados:

The test results of the ultra-micro nucleic acid analyzer show:

① The value of A260/A280 is above 1.8, e o valor de A260/A230 está acima 2.0, indicating that the purity of the extracted products is high.

② The nucleic acid extraction yield of 20mg wheat leaf samples is above 37 μg.

The results of agarose gel electrophoresis show:

① Wheat leaves all have bright bands and good integrity.

Para concluir: Using this kit to extract the genomic DNA of wheat leaf samples, the purity, yield and electrophoretic bands of the extracted products are all high.

Fornecedor

Xangai Lingjun Biotecnologia Co., Ltda.foi estabelecido em 2016 que é um fabricante profissional de materiais biomagnéticos e reagentes de extração de ácido nucleico.

Temos vasta experiência em extração e purificação de ácidos nucleicos, purificação de proteínas, separação celular, quimioluminescência, e outras áreas técnicas.

Nossos produtos são amplamente utilizados em muitos campos, como exames médicos, testes genéticos, pesquisa universitária, melhoramento genético, e assim por diante. Nós não apenas fornecemos produtos, mas também podemos realizar OEM, ODM, e outras necessidades. Se você tiver uma necessidade relacionada, não hesite em contactar-nos .