磁気ビーズ抽出技術で ASF 検査に革命を起こす

親愛なる大切なお客様, あなたはアフリカ豚コレラウイルスとの頭脳戦に巻き込まれていると聞きました? 心配しないでください。 “磁気釣竿” (凌軍 160011 磁気ビーズ), specially designed to hook DNA/RNA! With a simple (suck) そして (fling), the viral nucleic acids are caught in no time. Rest assured, your “Magnetic Fishing Kit” is all packed up and on its way to you— (SF Express) courier is sprinting your direction with it!

1つ、Magnetic bead-based nucleic acid extraction plays a critical role in the detection of African Swine Fever (ASF), with its high efficiency, 感度, and automation significantly enhancing the accuracy and speed of testing. Below are its specific roles and advantages:

1、Efficient Viral Nucleic Acid Extraction

High-Purity Nucleic Acids: The magnetic bead method specifically binds to nucleic acids (DNA/RNA), effectively isolating the genomic DNA of the African Swine Fever Virus (ASFV) while removing impurities such as proteins and lipids from the sample. This reduces PCR inhibitors and improves the reliability of subsequent detection.

Applicability to Complex Samples: It can process complex matrices such as blood, 組織 (脾臓, リンパ節), and environmental samples, making it particularly suitable for host samples where ASFV often lurks.

2、Enhanced Detection Sensitivity and Specificity

Detection of Low Viral Loads: The strong adsorption capacity of magnetic beads for trace nucleic acids improves the detection rate of low-concentration ASFV, which is especially important for monitoring early or asymptomatic infections.

Reduced False Negatives: Automated operations minimize human errors, and when combined with downstream detection methods such as quantitative PCR (qPCR), specificity can exceed 99%.

3、Rapid, High-Throughput Processing

Automated Platforms: The magnetic bead method can be integrated with automated extraction systems, processing dozens to hundreds of samples per run. This is suitable for large-scale screening (例えば, monitoring farms and slaughterhouses), reducing detection time to 1–2 hours.

Emergency Response: During ASF outbreaks, rapid nucleic acid extraction facilitates timely diagnosis and helps control transmission.

4、Biosafety Advantages

Closed-System Operation: The magnetic bead method is often performed in closed kits, reducing aerosol contamination and minimizing operators’ exposure to the virus. This aligns with the biosafety requirements for ASFV, a Biosafety Level 3 pathogen.

5、Compatibility with Multiple Detection Technologies
Extracted nucleic acids can be directly used for:

Real-Time Quantitative PCR (qPCR): The gold standard for ASFV detection, for which the magnetic bead method provides high-quality templates.

Isothermal Amplification Techniques (例えば, ランプ): Suitable for primary-level or field testing.

Sequencing Analysis: Used for viral genotyping and traceability studies.

Magnetic bead-based nucleic acid extraction is a core step in ASF detection. Its high efficiency and automation provide technical support for epidemic monitoring, early diagnosis, and prevention strategies. Combined with molecular techniques such as PCR, it significantly enhances the precision and response speed of ASF detection, making it indispensable for large-scale farming and cross-border quarantine.

二、Advantages of Lingjun Biological Magnetic Beads (製品番号. 160011)

As a core raw material in magnetic bead-based nucleic acid extraction reagents, biological magnetic beads play a decisive role in the nucleic acid extraction process. Lingjun Biological Magnetic Beads (160011) offer the following advantages:

1、Excellent operational performance with strong magnetic properties and short magnetic response time, reducing magnetic separation time.

2、Small particle size with a large surface area, enabling efficient nucleic acid binding and meeting the requirements for nucleic acid extraction from trace biological samples.

3、Suitable for automated extraction, reducing manual operation errors and meeting demands for high throughput and high sensitivity.

4、Excellent stability and batch-to-batch reproducibility.

5、Good storage stability: No aggregation after 1 year of storage at 2–8°C.

三つ、応用事例

The experimental results of Lingjun Biological 160011 magnetic beads combined with Lingjun Biological nucleic acid extraction reagents and a domestic manufacturer’s extraction reagents for the detection of swine fever virus in positive serum samples are as follows:

	Othere extraction reagents	Lingjun extraction reagents
サンプル	Ct值	Ct值
Serum-1	30.66	28.91
	30.81	29.03
Serum-2	21.55	19.41
	21.51	19.37
Serum-3	37.87	33.46
	37.02	33.55
Serum-4	30.25	28.45
	30.24	28.42
Serum-5	39.53	31.60
	37.51	31.82
Serum-6	22.46	20.73
	22.36	20.60
Serum-7	19.14	18.29
	19.31	18.42
Serum-8	33.14	31.24
	32.50	31.38

結果分析: For both high and low concentration samples, the CT values of Shanghai Lingjun’s extraction reagents were significantly earlier, indicating that the 160011 magnetic beads from Shanghai Lingjun, combined with Lingjun extraction reagents, delivered markedly better extraction efficiency and higher sensitivity.

サプライヤー

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光, その他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなくOEMも承ります, ODM, その他のニーズ. 関連するニーズがある場合, お気軽にお問い合わせください .