Manik Magnetik Lnjnbio untuk Kit Pengekstrakan DNA Genomik Darah Seluruh 231006(Kaedah Manik Magnet)

【Pengenalan Produk】

Whole blood sample under the action of guanidinium salt cells are lysed and release nucleic acid, through the specific adsorption of magnetic beads to separate the nucleic acid from other impurities in the sample, and then rinsed by the Wash Buffer, to remove the protein, sisa garam guanidinium, dll., dan akhirnya selepas elusi, to get a good purified nucleic acid, suitable for the extraction of DNA genomik from human whole blood, and the nucleic acid product obtained can be used for PCR, pengesanan dan analisis asid nukleik, in vitro diagnosis, pengesanan mutasi gen dan penjujukan, dll.

【Kelebihan produk】

1. For difficult to cleave samples (blood clot, dll.) can also realize the cleavage of the combination of one-step, do not need to pause in the middle to join other reagents

2. The extracted genomic DNA has good purity, hasil tinggi dan jalur elektroforesis lengkap.

3. Tidak perlu menambah proteinase K dalam proses perahan, and it can be transported and stored at room temperature.

4. Kecekapan pengekstrakan tinggi, keseluruhan proses pengekstrakan hanya mengambil masa kira-kira 15 minit.

5. The product does not contain phenol, kloroform dan reagen berbahaya lain, the use of the process is safe.

6. Strong applicability, boleh digunakan dengan pengeluar peralatan pengekstrakan asid nukleik automatik yang berbeza.

【Imej produk】

【Kes eksperimen】

Kes 1:

Experimental results of the same batch of reagents for extracting fresh blood samples from different healthy individuals:

Nombor sampel	Sampel	A260/A280	A260/A230	Conc.(ng/µL)
1	Blood sample No. 1	1.80	1.89	75.7
2	Blood sample No. 2	1.89	1.89	73.9
3	Blood sample No. 3	1.83	1.93	68.2
4	Blood sample No. 4	1.82	1.90	67.9
5	Blood sample No. 5	1.84	1.99	59.1

【The electropherogram is shown below】

Analisis keputusan: The human blood DNA extracted with this kit has good purity, high concentration and complete electrophoretic bands.

Kes 2：

Experimental results of different batches of reagents for extraction of the same pig blood sample:

Kit lot	A260/A280	A260/A230	Conc.(ng/µL)
Lot 1	1.87	1.90	157.5
Lot 1	1.87	1.96	137.9
Purata	1.87	1.93	147.7
Lot 2	1.88	1.92	132.6
Lot 2	1.88	1.99	140.4
Purata	1.88	1.96	136.5
Lot 3	1.89	1.95	141.2
Lot 3	1.89	1.91	141.7
Purata	1.89	1.93	141.4
Inter-lot average	1.88	1.94	141.87
Inter-lot CV	0.4%	0.7%	3.2%

Analisis keputusan：Extraction kits have low lot-to-lot variation and good stability.

Kes 3：

Experimental results of different batches of reagents for extraction of the same pig blood sample:

Kit lot	A260/A280	A260/A230	Conc.(ng/μL)
Lot 1	1.952	2.061	144.85
	1.96	2.061	116.55
	1.93	2.018	149.3
Purata	1.947	2.046	136.9
Lot 2	1.952	1.987	137.45
	1.947	1.983	137.9
	1.945	2.024	148.45
Purata	1.948	1.998	141.267
Inter-lot average	1.948	2.022	139.084
Inter-lot CV	0.02%	1.1%	1.5%

Analisis keputusan：Extraction kits have low lot-to-lot variation and good stability.

【Akawasan aplikasi】

The extracted and purified nucleic acids of this kit are suitable for various routine experiments such as enzyme digestion, PCR, pembinaan perpustakaan, Hibridisasi Selatan, and microarray hybridization and high-throughput sequencing.

Pengenalan Pembekal

Shanghai Lingjun Biotechnology Co., Ltd.ditubuhkan pada 2016 dan merupakan pengilang profesional bahan biomagnet dan reagen pengekstrakan asid nukleik.

Kami mempunyai pengalaman yang kaya dalam pengekstrakan dan penulenan asid nukleik, penulenan protein, pemisahan sel, chemiluminescence dan bidang teknikal lain.

Produk kami digunakan secara meluas dalam pelbagai bidang, seperti ujian perubatan, ujian genetik, penyelidikan universiti, pembiakan genetik, dan seterusnya. Kami bukan sahaja menyediakan produk tetapi juga boleh menjalankan OEM, ODM, dan keperluan lain. Jika anda mempunyai keperluan yang berkaitan, sila hubungi kami di sales01@lingjunbio.com.