Professionele fabrikant van biomagnetische kralen
Magnetische kralenmethode: Droge blad-DNA-extractie zo eenvoudig en efficiënt maken
Are you still troubled by the cumbersome steps of plant DNA extraction?
Centrifugation, extraction, toxic reagents like phenol-chloroform… Especially when dealing with precious dried leaf samples, do you always worry about low yield and poor results?
Now, here’s great news for research enthusiasts—the magnetic bead method for dry leaf DNA extraction is gaining popularity as a groundbreaking technique in molecular biology experiments, thanks to its revolutionary advantages!
1. What is the Magnetic Bead Method?
Imagine tiny “smart magnetic beads” that can accurately recognize and bind to DNA molecules. Simply mix the processed lysate of dried leaves with the magnetic beads, and the DNA will firmly adhere to the surface of the beads. Then, by bringing a magnet close, you can easily separate the “DNA-coated magnetic beads” from the liquid. After simple washing and elution steps, high-purity DNA is ready for use!
2. Why is It Particularly Suitable for Dry Leaves?
2.1Strong Tolerance:
Dry leaf cells are dehydrated, with more stubborn cell walls and secondary metabolites. The efficient lysis buffer used in the magnetic bead method can powerfully break down cell walls to release DNA, while effectively removing impurities such as polysaccharides and polyphenols.
2.2 High Yield and Purity:
Even if the sample has dried out, the high affinity of the magnetic beads for DNA ensures sufficient DNA yield, supporting stringent downstream applications like PCR and sequencing.
2.3 Ultimate Simplicity and Safety:
No centrifugation, filtration, or toxic reagents are required. The operational steps are greatly simplified, allowing multiple samples to be processed in 30–60 minutes. This truly achieves low cost, high efficiency, and high throughput.
2.4 Perfect Compatibility with Automation:
The entire “adsorption-washing-elution” process is ideally suited for automated nucleic acid extraction instruments, freeing your hands from manual work, ensuring stable and reliable results, and eliminating human error.
3. Wide Range of Applications
Whether it’s precious specimens collected during field investigations, archived samples from seed banks, or large-scale screening in agricultural breeding, the magnetic bead method can handle them all with ease, making your research journey smoother.
- Application Case: Lingjun Bio 160014 Magnetic Beads with Lingjun Bio Plant Seed DNA Extraction Reagent
The experimental results are as follows:
| Nummer | Steekproef | Sampling Amount | A260/A280 | A260/A230 | Conc.(van/ul) |
| 1 | Dried corn leaves | 5mg | 1.822 | 1.788 | 175.6 |
| 2 | Dried corn leaves | 5mg | 1.819 | 1.766 | 208 |
| 3 | Dried corn leaves | 10mg | 1.815 | 1.777 | 294.1 |
| 4 | Dried corn leaves | 10mg | 1.803 | 1.728 | 307.85 |
| 5 | Dried dandelion leaves | 5mg | 1.847 | 1.856 | 205.45 |
| 6 | Dried dandelion leaves | 5mg | 1.832 | 1.806 | 217.05 |
Electrophoresis results:
Results Analysis:
Tests with an ultra-micro nucleic acid analyzer showed: A260/A280 ratios above 1.8, and A260/A230 ratios above 1.7, indicating high purity of the extracted products.
Agarose gel electrophoresis results revealed: Bright and intact bands for different dry leaf plant samples, demonstrating good integrity.
Samengevat: The extraction of various dry leaf plant samples yielded products with high purity, hoge opbrengst, and intact electrophoresis bands.
5. Conclusion
The magnetic bead DNA extraction technology, with its distinct features of speed, efficiency, safety, and automation, perfectly addresses the pain points of dry leaf DNA extraction. It is not just a technological advancement but also a revolution in research efficiency.
Choosing the magnetic bead method means opting for a smarter and more effortless research experience!
Leverancier
Shanghai Lingjun Biotechnologie Co., Ltd.werd opgericht in 2016 dat een professionele fabrikant is van biomagnetische materialen en reagentia voor nucleïnezuurextractie.
We hebben een rijke ervaring in de extractie en zuivering van nucleïnezuren, eiwit zuivering, cel scheiding, chemiluminescentie, en andere technische gebieden.
Onze producten worden op veel gebieden veel gebruikt, zoals medische testen, genetische testen, universitair onderzoek, genetische veredeling, enzovoort. Wij leveren niet alleen producten, maar kunnen ook OEM ondernemen, ODM, en andere behoeften. Als u een gerelateerde behoefte heeft, Neem gerust contact met ons op .
