Fabricante profesional de perlas biomagnéticas
Método de cuentas magnéticas: Cómo hacer que la extracción de ADN de hojas secas sea tan simple y eficiente
¿Todavía te preocupan los engorrosos pasos de la extracción del ADN de las plantas??
Centrifugación, extracción, reactivos tóxicos como el fenol-cloroformo… Especialmente cuando se trata de preciosas muestras de hojas secas., do you always worry about low yield and poor results?
Ahora, here’s great news for research enthusiasts—the magnetic bead method for dry leaf DNA extraction is gaining popularity as a groundbreaking technique in molecular biology experiments, thanks to its revolutionary advantages!
1. What is the Magnetic Bead Method?
Imagine tiny “smart magnetic beads” that can accurately recognize and bind to DNA molecules. Simply mix the processed lysate of dried leaves with the magnetic beads, and the DNA will firmly adhere to the surface of the beads. Entonces, by bringing a magnet close, you can easily separate the “DNA-coated magnetic beads” from the liquid. After simple washing and elution steps, high-purity DNA is ready for use!
2. Why is It Particularly Suitable for Dry Leaves?
2.1Strong Tolerance:
Dry leaf cells are dehydrated, with more stubborn cell walls and secondary metabolites. The efficient lysis buffer used in the magnetic bead method can powerfully break down cell walls to release DNA, while effectively removing impurities such as polysaccharides and polyphenols.
2.2 Alto rendimiento y pureza:
Even if the sample has dried out, the high affinity of the magnetic beads for DNA ensures sufficient DNA yield, supporting stringent downstream applications like PCR and sequencing.
2.3 Ultimate Simplicity and Safety:
No centrifugation, filtration, or toxic reagents are required. The operational steps are greatly simplified, allowing multiple samples to be processed in 30–60 minutes. This truly achieves low cost, high efficiency, y alto rendimiento.
2.4 Perfect Compatibility with Automation:
The entire “adsorción-lavado-elución” process is ideally suited for automated nucleic acid extraction instruments, freeing your hands from manual work, ensuring stable and reliable results, and eliminating human error.
3. Wide Range of Applications
Whether it’s precious specimens collected during field investigations, archived samples from seed banks, or large-scale screening in agricultural breeding, the magnetic bead method can handle them all with ease, making your research journey smoother.
- Caso de aplicación: Biografía de Lingjun 160014 Magnetic Beads with Lingjun Bio Plant Seed DNA Extraction Reagent
The experimental results are as follows:
| Número | Muestra | Sampling Amount | A260/A280 | A260/A230 | concentrado.(de/ul) |
| 1 | Dried corn leaves | 5mg | 1.822 | 1.788 | 175.6 |
| 2 | Dried corn leaves | 5mg | 1.819 | 1.766 | 208 |
| 3 | Dried corn leaves | 10mg | 1.815 | 1.777 | 294.1 |
| 4 | Dried corn leaves | 10mg | 1.803 | 1.728 | 307.85 |
| 5 | Dried dandelion leaves | 5mg | 1.847 | 1.856 | 205.45 |
| 6 | Dried dandelion leaves | 5mg | 1.832 | 1.806 | 217.05 |
Electrophoresis results:
Análisis de resultados:
Tests with an ultra-micro nucleic acid analyzer showed: A260/A280 ratios above 1.8, and A260/A230 ratios above 1.7, indicating high purity of the extracted products.
Agarose gel electrophoresis results revealed: Bright and intact bands for different dry leaf plant samples, demonstrating good integrity.
En resumen: The extraction of various dry leaf plant samples yielded products with high purity, alto rendimiento, y bandas de electroforesis intactas.
5. Conclusión
The magnetic bead DNA extraction technology, with its distinct features of speed, eficiencia, seguridad, y automatización, perfectly addresses the pain points of dry leaf DNA extraction. It is not just a technological advancement but also a revolution in research efficiency.
Choosing the magnetic bead method means opting for a smarter and more effortless research experience!
Proveedor
Shanghai Lingjun Biotecnología Co., Limitado.se estableció en 2016 que es un fabricante profesional de materiales biomagnéticos y reactivos de extracción de ácidos nucleicos..
Tenemos una rica experiencia en extracción y purificación de ácidos nucleicos., purificación de proteínas, separación celular, quimioluminiscencia, y otros campos técnicos.
Nuestros productos son ampliamente utilizados en muchos campos., como pruebas médicas, pruebas genéticas, investigación universitaria, mejoramiento genético, etcétera. No solo proporcionamos productos sino que también podemos realizar OEM, ODM, y otras necesidades. Si tienes una necesidad relacionada, no dude en contactarnos .
