마그네틱 비드 방식: 마른 잎 DNA 추출을 매우 간단하고 효율적으로 만들기

아직도 식물 DNA 추출의 번거로운 단계 때문에 고민이신가요??

원심분리, 추출, 페놀-클로로포름과 같은 독성 시약… 특히 귀중한 말린 잎 샘플을 다룰 때, do you always worry about low yield and poor results?

지금, here’s great news for research enthusiasts—the magnetic bead method for dry leaf DNA extraction is gaining popularity as a groundbreaking technique in molecular biology experiments, thanks to its revolutionary advantages!

1. What is the Magnetic Bead Method?

Imagine tiny “smart magnetic beads” that can accurately recognize and bind to DNA molecules. Simply mix the processed lysate of dried leaves with the magnetic beads, and the DNA will firmly adhere to the surface of the beads. 그 다음에, by bringing a magnet close, you can easily separate the “DNA-coated magnetic beads” from the liquid. After simple washing and elution steps, high-purity DNA is ready for use!

2. Why is It Particularly Suitable for Dry Leaves?

2.1Strong Tolerance:

Dry leaf cells are dehydrated, with more stubborn cell walls and secondary metabolites. The efficient lysis buffer used in the magnetic bead method can powerfully break down cell walls to release DNA, while effectively removing impurities such as polysaccharides and polyphenols.

2.2 높은 수율과 순도:

Even if the sample has dried out, the high affinity of the magnetic beads for DNA ensures sufficient DNA yield, supporting stringent downstream applications like PCR and sequencing.

2.3 Ultimate Simplicity and Safety:

No centrifugation, filtration, or toxic reagents are required. The operational steps are greatly simplified, allowing multiple samples to be processed in 30–60 minutes. This truly achieves low cost, 고효율, 높은 처리량.

2.4 Perfect Compatibility with Automation:

The entire “adsorption-washing-elution” process is ideally suited for automated nucleic acid extraction instruments, freeing your hands from manual work, ensuring stable and reliable results, and eliminating human error.

3. Wide Range of Applications

Whether it’s precious specimens collected during field investigations, archived samples from seed banks, or large-scale screening in agricultural breeding, the magnetic bead method can handle them all with ease, making your research journey smoother.

• 적용사례: 링준바이오 160014 Magnetic Beads with Lingjun Bio Plant Seed DNA Extraction Reagent

The experimental results are as follows:

숫자	견본	Sampling Amount	A260/A280	A260/A230	농도.(/ul의)
1	Dried corn leaves	5mg	1.822	1.788	175.6
2	Dried corn leaves	5mg	1.819	1.766	208
3	Dried corn leaves	10mg	1.815	1.777	294.1
4	Dried corn leaves	10mg	1.803	1.728	307.85
5	Dried dandelion leaves	5mg	1.847	1.856	205.45
6	Dried dandelion leaves	5mg	1.832	1.806	217.05

Electrophoresis results:

결과 분석:

Tests with an ultra-micro nucleic acid analyzer showed: A260/A280 ratios above 1.8, and A260/A230 ratios above 1.7, 추출된 제품의 순도가 높다는 것을 나타냅니다..

Agarose gel electrophoresis results revealed: Bright and intact bands for different dry leaf plant samples, demonstrating good integrity.
요약하면: The extraction of various dry leaf plant samples yielded products with high purity, 높은 수율, 손상되지 않은 전기영동 밴드.

5. 결론

The magnetic bead DNA extraction technology, with its distinct features of speed, efficiency, 안전, 자동화, perfectly addresses the pain points of dry leaf DNA extraction. It is not just a technological advancement but also a revolution in research efficiency.

Choosing the magnetic bead method means opting for a smarter and more effortless research experience!

공급자

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광, 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련된 필요사항이 있는 경우, 저희에게 연락하게 자유롭게 느끼십시오 .