Como extrair eficientemente DNA/RNA de soro e plasma?

Introdução

No campo do diagnóstico molecular, a extração de ácido nucleico viral é uma etapa fundamental na obtenção de informações importantes de amostras biológicas, como soro e plasma. Traditional nucleic acid extraction methods are often cumbersome, time-consuming and limited in efficiency.

Based on this demand, Xangai Lingjun Biotecnologia Co., Ltda. develops and produces 160025 esferas magnéticas à base de sílica (average particle size of 800nm) to meet the special needs of customers who are able to amplify directly with magnetic beads.

1. Product recommendation

160025 silicon-based magnetic beads with high adsorption rate, good stability, no ferrous ion leakage and so on.

160025
Grupo ativo	Si-OH
Matriz de contas magnéticas	Sílica superparamagnética
Mean particle diameter	800nm
Value of saturated magnetic	60±5emu/g

• With an average particle size of 800 nm, this magnetic bead has excellent levitation properties and magnetic response speed. Its magnetic saturation strength reaches 60±5 emu/g, ensuring efficient magnetic separation.
• The product adopts silica hydroxyl (Si-OH) active group, silica magnetic bead matrix, which has excellent nucleic acid adsorption ability. A small amount of magnetic beads can obtain the target nucleic acid, and the amount of magnetic beads does not affect PCR amplification.
• What’s more, the magnetic beads can lyse pathogens at room temperature without heating, boiling or centrifugation, which greatly simplifies the operation process.

2. Simple operation, no elution, direct amplification with magnetic beads

No centrifugation is required during the experiment, magnetic beads do not need to elute nucleic acids, and PCR amplification can be carried out with magnetic beads, which greatly simplifies the operation steps.

This method not only reduces the operation time, but also reduces the risk of cross-contamination and improves the reliability and reproducibility of the experimental results.

The whole extraction process only takes about 30 minutos, and the extracted viral nucleic acid has good purity and high yield, which can be directly carried out various downstream experiments.

3. Wide range of applications for a variety of sample types, including serum, plasma, cotonetes, urina, etc..

Viral nucleic acid can be extracted from serum, plasma, cotonetes, urina, faeces, blister fluid and other samples. The extracted nucleic acid has good purity and high yield, and can be directly used for downstream experiments such as enzyme digestion, PCR, fluorescence quantitative PCR and library construction.

LnjnBio has developed various types of micro and nano magnetic microsphere series, incluindo fluido magnético, monodisperse silicon-based magnetic spheres, polydisperse silicon-based magnetic spheres and so on.

In order to match the automated workstation and automatic nucleic acid extractor, the company has also developed various magnetic bead method nucleic acid extraction kit series.

4.Rapid growth of viral nucleic acid extraction market

With the rapid development of molecular diagnostic technology, there is a growing demand for efficient and accurate nucleic acid extraction technology. Our products have been widely used in many fields such as university research, germplasm resources and genetic breeding.

O 160025 silica-based magnetic beads can complete the whole extraction process in about 30 minutos, and the extracted viral nucleic acid has good purity and high yield, which can be directly used in various downstream experiments.

No futuro, with the continuous development of molecular diagnostic technology, Leader Bio will continue to develop more innovative products and provide better technical services for the life science field.

Fornecedor

Xangai Lingjun Biotecnologia Co., Ltda.foi estabelecido em 2016 que é um fabricante profissional de materiais biomagnéticos e reagentes de extração de ácido nucleico.

Temos vasta experiência em extração e purificação de ácidos nucleicos, purificação de proteínas, separação celular, quimioluminescência, e outras áreas técnicas.

Nossos produtos são amplamente utilizados em muitos campos, como exames médicos, testes genéticos, pesquisa universitária, melhoramento genético, e assim por diante. Nós não apenas fornecemos produtos, mas também podemos realizar OEM, ODM, e outras necessidades. Se você tiver uma necessidade relacionada, não hesite em contactar-nos .