Kontribusi teknologi ekstraksi genom tanaman berbasis metode manik magnetik terhadap penelitian transgenik pertanian di Tiongkok

1. Mempercepat penelitian dan pengembangan tanaman hasil rekayasa genetika

A.Analisis genotipe throughput tinggi

Ekstraksi DNA yang cepat: Metode manik magnetik dapat menyelesaikan ekstraksi DNA jaringan tanaman (seperti daun, benih) di dalam 30 menit, yang 3-5 kali lebih cepat dibandingkan metode CTAB tradisional. Sangat cocok untuk penyaringan awal (generasi T0) dan identifikasi selanjutnya (Generasi T1-T3) tanaman transgenik skala besar.

DNA dengan kemurnian tinggi: DNA diekstraksi dengan manik-manik magnetik (A260/A280 ≈ 1.8-2.0) dapat langsung digunakan untuk verifikasi pengeditan PCR dan CRISPR, mengurangi hasil negatif palsu.

B. Pemrosesan sampel yang rumit untuk polifenol dan adaptasi tanaman polisakarida:

 Untuk tanaman seperti kapas dan padi yang kaya akan inhibitor, manik-manik magnetik silanol dapat secara efektif menyerap DNA dan menghilangkan polisakarida dan polifenol, sehingga meningkatkan efisiensi dan akurasi transformasi.  

2. Pengawasan yang Tepat Dan Pengujian Keamanan

a.Detection of Low-Concentration Target Genetically Modified Components: The magnetic bead method can extract trace amounts of genetically modified components (such as the CaMV 35S promoter) from processed foods (such as soybean oil), with a detection limit of up to 0.1% (in accordance with EU standards), supporting market supervision.

Standardized Process: It is compatible with the “Detection of Components in Genetically Modified Plants and Their Products” issued by the Ministry of Agriculture and Rural Affairs (such as NY/T 672-2023), ensuring the repeatability of the test results.

B.Field Monitoring

Rapid on-site testing: The portable magnetic bead extraction instrument can complete DNA extraction in the field. Combined with LAMP technology, it enables a 1-hour rapid detection of genetically modified crops, preventing the spread of illegal cultivation.

3. Promote the upgrading of molecular breeding technology

A.Marker-assisted breeding

Improvement of SNP typing efficiency: DNA extracted by magnetic beads can be directly used on high-throughput SNP chips, accelerating the screening of markers for insect resistance and disease resistance traits.

Single-cell genomics: Magnetic bead microfluidic technology can separate the genome of individual plant cells, which can be used for haploid breeding or analysis of off-target effects.

B.Compliance of Genetically Modified Crops

Verification of absence of exogenous DNA: The combination of magnetic bead method and qPCR can accurately detect whether plasmid DNA (such as Agrobacterium T-DNA) is residual in CRISPR-edited crops, meeting the requirements of the “Guidelines for Safety Evaluation of Agricultural Transgenic Organisms”.

• Synergy of the industrial chain

Phase	Contributions of Magnetic Bead Method
Seed enterprises	Quickly identify the purity of parent seeds to prevent contamination of conventional varieties by transgenic pollen
Testing institutions	Realize high-throughput detection in 96-well plates, reducing the cost per sample (such as customs import and export screening)
Farmers	Identify genuine and fake transgenic seeds through low-cost rapid detection technology.

5. Challenges and Improvement Directions

Pengendalian Biaya: Manik-manik magnetik domestik (such as Shanghai Lingjun) cost one-third of the imported ones. Further price reduction is possible for large-scale applications.  

Automation integration: Promote the deep compatibility between the magnetic bead method and domestic automated equipment to reduce human errors.  

Kasus: Detection of Genetically Modified Soybeans

1. Ekstraksi: DNA was extracted from soybean powder using the magnetic bead method (100mg sample → 2μg DNA).

2. Deteksi: The CP4-EPSPS gene was detected by qPCR. A Ct value < 30 was considered positive.

3.Makna: This method supports the on-site inspection of imported genetically modified soybeans in China. The detection rate increased to 99.7% di dalam 2023.

Angka 1：Kit Ekstraksi DNA/RNA Tanaman

The magnetic bead-based plant genome extraction technology, through three major paths of accelerating research and development, precise supervision, and empowering breeding, has become a key supporting tool for the development of transgenic agriculture in China. Di masa depan, with the improvement of domestic magnetic bead performance and the popularization of automation, the technical threshold will be further lowered, promoting the safe and controllable industrialization of transgenic crops.

Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 yang merupakan produsen profesional bahan biomagnetik dan reagen ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, kimialuminesensi, dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami .