使用期限が切れた磁気ビーズ法ベースの核酸抽出試薬はまだ使用できますか?

以下の要素を総合的に考慮し、リスクを慎重に評価する必要があります:

1. The impact of expired reagents

Magnetic bead performance degradation: 磁気ビーズが凝集する可能性があります, degrade or lose their surface modification groups, resulting in reduced binding efficiency and affecting the yield or purity of nucleic acids.

バッファの不活性化: プロテイナーゼK, lysis buffer or washing buffer may become ineffective (such as pH changes, precipitation and sedimentation), leading to incomplete lysis or residue of impurities.

Unreliable results: The extracted nucleic acids may be degraded, contaminated or have insufficient concentration, affecting downstream experiments (such as PCR, 順序付け).

2. Steps for evaluating usability

Visual inspection: Check if the reagent has any precipitation, turbidity, color change or stratification (such as when magnetic beads cannot be resuspended after precipitation).

Performance test: Extract with a standard sample of known concentration (such as cultured cells or standard DNA), and compare the yield, 純度 (A260/A280) and downstream application effect (such as amplification efficiency) of the fresh reagent.

Set up positive and negative controls to verify the specificity of the reagent.

Key experimental requirements: If the nucleic acid quality is extremely demanding (such as single-cell sequencing, low-abundance samples), it is recommended to directly replace with a new reagent.

3. Potential Risks

False negative/false positive results: Especially for diagnostic or clinical samples, they may lead to incorrect conclusions.

Cost of repeated experiments: If the data becomes unreliable due to the failure of reagents, it may waste more time and samples.

Sample loss: Valuable or limited samples (such as clinical biopsies, rare microorganisms) should not be riskily processed with expired reagents.

• Suggestions for non-critical experiments:

If the test results are acceptable, they can be temporarily used for preliminary experiments or teaching purposes.

Critical experiments: It is essential to replace expired reagents to ensure the reliability of the results.

Storage in aliquots: If the reagents are prone to deterioration, purchase them and then aliquot and freeze store them (to avoid repeated freezing and thawing), which can extend their stability. まとめ

Expired reagents may still be effective, but they need to be rigorously verified. After weighing the risks, it is recommended to prioritize the use of reagents that are within their expiration dates, especially when they are involved in scientific research publications, clinical diagnoses, or precious samples.

サプライヤー

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出試薬の専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光, その他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなくOEMも承ります, ODM, その他のニーズ. 関連するニーズがある場合, お気軽にお問い合わせください .