유효기간이 지난 마그네틱비드법 기반 핵산추출시약을 계속 사용할 수 있나요??

다음 요소를 종합적으로 고려하고 위험을 신중하게 평가해야 합니다.:

1. The impact of expired reagents

Magnetic bead performance degradation: 자기 비드가 응집될 수 있습니다., degrade or lose their surface modification groups, resulting in reduced binding efficiency and affecting the yield or purity of nucleic acids.

Buffer Inactivation: 단백질분해효소 K, lysis buffer or washing buffer may become ineffective (such as pH changes, precipitation and sedimentation), leading to incomplete lysis or residue of impurities.

Unreliable results: The extracted nucleic acids may be degraded, contaminated or have insufficient concentration, affecting downstream experiments (PCR과 같은, 시퀀싱).

2. Steps for evaluating usability

Visual inspection: Check if the reagent has any precipitation, turbidity, color change or stratification (such as when magnetic beads cannot be resuspended after precipitation).

Performance test: Extract with a standard sample of known concentration (such as cultured cells or standard DNA), and compare the yield, purity (A260/A280) and downstream application effect (such as amplification efficiency) of the fresh reagent.

Set up positive and negative controls to verify the specificity of the reagent.

Key experimental requirements: If the nucleic acid quality is extremely demanding (such as single-cell sequencing, low-abundance samples), it is recommended to directly replace with a new reagent.

3. Potential Risks

위음성/위양성 결과: Especially for diagnostic or clinical samples, they may lead to incorrect conclusions.

Cost of repeated experiments: If the data becomes unreliable due to the failure of reagents, it may waste more time and samples.

Sample loss: Valuable or limited samples (such as clinical biopsies, rare microorganisms) should not be riskily processed with expired reagents.

• Suggestions for non-critical experiments:

If the test results are acceptable, they can be temporarily used for preliminary experiments or teaching purposes.

Critical experiments: It is essential to replace expired reagents to ensure the reliability of the results.

Storage in aliquots: If the reagents are prone to deterioration, purchase them and then aliquot and freeze store them (to avoid repeated freezing and thawing), which can extend their stability. 요약

Expired reagents may still be effective, but they need to be rigorously verified. After weighing the risks, it is recommended to prioritize the use of reagents that are within their expiration dates, especially when they are involved in scientific research publications, clinical diagnoses, or precious samples.

공급자

상하이 링쥔 생명공학 유한회사, 주식회사에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

핵산 추출 및 정제 분야에서 풍부한 경험을 보유하고 있습니다., 단백질 정제, 세포 분리, 화학발광, 및 기타 기술 분야.

우리의 제품은 많은 분야에서 널리 사용됩니다., 의료 테스트와 같은, 유전자 검사, 대학 연구, 유전자 육종, 등. 우리는 제품을 제공할 뿐만 아니라 OEM도 수행할 수 있습니다., ODM, 그리고 다른 필요. 관련된 필요사항이 있는 경우, 저희에게 연락하게 자유롭게 느끼십시오 .