Universal Total RNA Extraktioun Kit: The Key to Preventing RNA Degradation in Routine Experiments

1. Produit Aféierung:

The universal total RNA extraction reagent is suitable for rapidly and efficiently extracting total RNA from various tissue samples such as fresh plants, animals, fungi, and cells. The entire process is easy to operate and is particularly suitable for automated nucleic acid extraction platforms. Wärend dem Extraktiounsprozess, the sample releases nucleic acids under the action of the lysis buffer. The nano-magnetic beads selectively adsorb the nucleic acids under the action of the binding buffer, separating the nucleic acids from other impurities in the sample. Then rinse with the washing solution to remove proteins, polysaccharides, anorganesch Salzionen, etc. Endlech, high-quality RNA was obtained through elution. This product does not require toxic reagents such as phenol and chloroform. The extracted RNA has a high yield, héich Rengheet, stabil an zouverlässeg Qualitéit, a kann op verschidde downstream molekulare Biologie Experimenter applizéiert ginn.

2.Product case

Fall 1: RNA extraction experiments were conducted on multiple fresh tissue samples, an d'Resultater sinn wéi follegt:

Prouf Zuel	Sample name	Sampling quantity	A260/A280	A260/A230	Konz.(ng/μL)	Ausgang(μg)
1	Baby cabbage leaves	10mg	2.073	2.112	109.35	10.9
2	Romaine lettuce leaves		2.01	1.974	114.8	11.5
3	Chicken feather vegetable leaves		2.109	2.045	79.45	8
4	Pea seedlings		2.053	1.972	108.75	10.9

D'Elektrophoresediagramm ass wéi follegt:

Fall 2: A total RNA extraction experiment was conducted using multiple fresh tissue samples, an d'Resultater sinn wéi follegt:

Prouf Zuel	Sample name	Sampling quantity	A260/A280	A260/A230	Konz.(ng/μL)	Ausgang(μg)
1	Spinat Blieder	10mg	1.996	2.112	113.35	11.3
2	Koriander Blieder		1.955	1.936	58.95	6
3	Chinese cabbage leaves		2.001	2.102	130.35	13
4	Sellerie Blieder		1.999	2.142	77.55	7.8
5	Schwäin Liewer	5mg	1.884	1.713	88.75	8.9
6	Schwäin Liewer	10mg	1.872	1.688	112.05	11.2

D'Elektrophoresediagramm ass wéi follegt:

Fall 3: A total RNA extraction experiment was conducted using multiple fresh tissue samples, an d'Resultater sinn wéi follegt:

Prouf Zuel	Sample name	Sampling quantity	A260/A280	A260/A230	Konz.(ng/μL)	Ausgang(μg)
1	Spinat Blieder	10mg	1.996	2.112	113.35	11.3
2	Chinese cabbage leaves	10mg	2.001	2.102	130.35	13
3	Sellerie Blieder	10mg	1.999	2.142	77.55	7.8
4	Koriander Blieder	20mg	2.113	2.423	77.8	7.8
5	Koriander Blieder	20mg	2.03	2.116	203.75	20.4
6	Shiitake mushroom	10mg	2.171	2.68	65.8	6.6
7	Enoki mushrooms	10mg	2.073	1.969	44.45	4.5
8	White jade mushroom	20mg	2.157	2.517	56.8	5.7
9	Wäiss Champignon	20mg	2.111	2.42	118.7	11.9
10	Schwäin	10mg	2.041	2.011	32.2	3.2
11	Schwäin Liewer	5mg	1.884	1.713	88.75	8.9
12	Schwäin Liewer	10mg	1.872	1.708	112.05	11.2

D'Elektrophoresediagramm ass wéi follegt:

3.Experimental pain points

Den universellen Total RNA Extraktiounsreagens (magnetesch Perle Method) has solved multiple experimental pain points in traditional RNA extraction by optimizing the technology and process, and is particularly suitable for complex samples and high-throughput requirements. The following are its core advantages and the corresponding solutions to pain points:

4.Product advantages

4.1 It has strong versatility and is suitable for extracting total RNA from various tissue samples such as animals, Planzen, fungi, and cells.

4.2 It is applicable to the extraction of total RNA from trace samples, trace tissue samples and cultured cells (a small number of cells) Echantillon.

4.3 The operation is simple and convenient. The magnetic bead method can be combined with an automatic nucleic acid extractor to achieve automated extraction. Lysis and lysis can be carried out in one step, making the extraction highly efficient.

4.4 No need for repeated centrifugation, d'Integritéit vum extrahéierten RNA assuréieren.

Fournisseur

Shanghai Lingjun Biotechnology Co., Ltd., Ltd.gouf etabléiert an 2016 deen e professionnelle Hiersteller vu biomagnetesche Materialien an Nukleinsäure Extraktiounsreagenz ass.

Mir hu räich Erfahrung an Nukleinsäure Extraktioun an Offäll, Protein Reinigung, Zell Trennung, Chemilumineszenz, an aner technesch Beräicher.

Eis Produkter gi wäit a ville Beräicher benotzt, wéi medizinesch Tester, genetesch Tester, Universitéit Fuerschung, genetesch Zucht, an esou weider. Mir bidden net nëmmen Produkter, awer och kënnen OEM ënnerhuelen, ODM, an aner Besoinen. Wann Dir e verbonne Besoin hutt, weg fillen gratis eis ze kontaktéieren .