Kit Pengekstrakan RNA Jumlah Sejagat: The Key to Preventing RNA Degradation in Routine Experiments

1. Pengenalan Produk:

The universal total RNA extraction reagent is suitable for rapidly and efficiently extracting total RNA from various tissue samples such as fresh plants, animals, kulat, and cells. The entire process is easy to operate and is particularly suitable for automated nucleic acid extraction platforms. Semasa proses perahan, the sample releases nucleic acids under the action of the lysis buffer. The nano-magnetic beads selectively adsorb the nucleic acids under the action of the binding buffer, mengasingkan asid nukleik daripada kekotoran lain dalam sampel. Then rinse with the washing solution to remove proteins, polisakarida, ion garam tak organik, dll. Akhirnya, high-quality RNA was obtained through elution. Produk ini tidak memerlukan reagen toksik seperti fenol dan kloroform. The extracted RNA has a high yield, kesucian yang tinggi, kualiti yang stabil dan boleh dipercayai, dan boleh digunakan untuk pelbagai eksperimen biologi molekul hiliran.

2.Product case

Kes 1: RNA extraction experiments were conducted on multiple fresh tissue samples, dan hasilnya adalah seperti berikut:

Nombor sampel	Sample name	Sampling quantity	A260/A280	A260/A230	Conc.(ng/μL)	Keluaran（μg）
1	Baby cabbage leaves	10mg	2.073	2.112	109.35	10.9
2	Romaine lettuce leaves		2.01	1.974	114.8	11.5
3	Chicken feather vegetable leaves		2.109	2.045	79.45	8
4	Pea seedlings		2.053	1.972	108.75	10.9

Gambar rajah elektroforesis adalah seperti berikut:

Kes 2: A total RNA extraction experiment was conducted using multiple fresh tissue samples, dan hasilnya adalah seperti berikut:

Nombor sampel	Sample name	Sampling quantity	A260/A280	A260/A230	Conc.(ng/μL)	Keluaran（μg）
1	Daun bayam	10mg	1.996	2.112	113.35	11.3
2	Daun ketumbar		1.955	1.936	58.95	6
3	Chinese cabbage leaves		2.001	2.102	130.35	13
4	Daun saderi		1.999	2.142	77.55	7.8
5	Hati babi	5mg	1.884	1.713	88.75	8.9
6	Hati babi	10mg	1.872	1.688	112.05	11.2

Gambar rajah elektroforesis adalah seperti berikut:

Kes 3: A total RNA extraction experiment was conducted using multiple fresh tissue samples, dan hasilnya adalah seperti berikut:

Nombor sampel	Sample name	Sampling quantity	A260/A280	A260/A230	Conc.(ng/μL)	Keluaran（μg）
1	Daun bayam	10mg	1.996	2.112	113.35	11.3
2	Chinese cabbage leaves	10mg	2.001	2.102	130.35	13
3	Daun saderi	10mg	1.999	2.142	77.55	7.8
4	Daun ketumbar	20mg	2.113	2.423	77.8	7.8
5	Daun ketumbar	20mg	2.03	2.116	203.75	20.4
6	Shiitake mushroom	10mg	2.171	2.68	65.8	6.6
7	Enoki mushrooms	10mg	2.073	1.969	44.45	4.5
8	White jade mushroom	20mg	2.157	2.517	56.8	5.7
9	Cendawan putih	20mg	2.111	2.42	118.7	11.9
10	daging babi	10mg	2.041	2.011	32.2	3.2
11	Hati babi	5mg	1.884	1.713	88.75	8.9
12	Hati babi	10mg	1.872	1.708	112.05	11.2

Gambar rajah elektroforesis adalah seperti berikut:

3.Experimental pain points

Reagen pengekstrakan total RNA universal (kaedah manik magnetik) has solved multiple experimental pain points in traditional RNA extraction by optimizing the technology and process, and is particularly suitable for complex samples and high-throughput requirements. The following are its core advantages and the corresponding solutions to pain points:

4.Product advantages

4.1 It has strong versatility and is suitable for extracting total RNA from various tissue samples such as animals, tumbuhan, kulat, and cells.

4.2 It is applicable to the extraction of total RNA from trace samples, trace tissue samples and cultured cells (a small number of cells) sampel.

4.3 The operation is simple and convenient. The magnetic bead method can be combined with an automatic nucleic acid extractor to achieve automated extraction. Lysis and lysis can be carried out in one step, making the extraction highly efficient.

4.4 No need for repeated centrifugation, memastikan integriti RNA yang diekstrak.

Pembekal

Shanghai Lingjun Biotechnology Co., Ltd.ditubuhkan pada 2016 yang merupakan pengilang profesional bahan biomagnet dan reagen pengekstrakan asid nukleik.

Kami mempunyai pengalaman yang kaya dalam pengekstrakan dan penulenan asid nukleik, penulenan protein, pemisahan sel, chemiluminescence, dan bidang teknikal yang lain.

Produk kami digunakan secara meluas dalam pelbagai bidang, seperti ujian perubatan, ujian genetik, penyelidikan universiti, pembiakan genetik, dan seterusnya. Kami bukan sahaja menyediakan produk tetapi juga boleh menjalankan OEM, ODM, dan keperluan lain. Jika anda mempunyai keperluan yang berkaitan, sila hubungi kami .