Universal Total RNA Extraction Kit: Nøglen til at forhindre RNA-nedbrydning i rutineeksperimenter

1. Produktintroduktion:

The universal total RNA extraction reagent is suitable for rapidly and efficiently extracting total RNA from various tissue samples such as fresh plants, animals, svampe, and cells. The entire process is easy to operate and is particularly suitable for automated nucleic acid extraction platforms. During the extraction process, the sample releases nucleic acids under the action of the lysis buffer. The nano-magnetic beads selectively adsorb the nucleic acids under the action of the binding buffer, separating the nucleic acids from other impurities in the sample. Then rinse with the washing solution to remove proteins, polysaccharider, uorganiske saltioner, osv. Endelig, high-quality RNA was obtained through elution. This product does not require toxic reagents such as phenol and chloroform. The extracted RNA has a high yield, høj renhed, stabil og pålidelig kvalitet, og kan anvendes til forskellige nedstrøms molekylærbiologiske eksperimenter.

2.Product case

Sag 1: RNA extraction experiments were conducted on multiple fresh tissue samples, og resultaterne er som følger:

Prøvenummer	Sample name	Sampling quantity	A260/A280	A260/A230	Konc.(ng/μL)	Produktion(μg)
1	Baby cabbage leaves	10mg	2.073	2.112	109.35	10.9
2	Romaine lettuce leaves		2.01	1.974	114.8	11.5
3	Chicken feather vegetable leaves		2.109	2.045	79.45	8
4	Pea seedlings		2.053	1.972	108.75	10.9

Elektroforesediagrammet er som følger:

Sag 2: A total RNA extraction experiment was conducted using multiple fresh tissue samples, og resultaterne er som følger:

Prøvenummer	Sample name	Sampling quantity	A260/A280	A260/A230	Konc.(ng/μL)	Produktion(μg)
1	Spinatblade	10mg	1.996	2.112	113.35	11.3
2	Koriander blade		1.955	1.936	58.95	6
3	Chinese cabbage leaves		2.001	2.102	130.35	13
4	Bladselleri		1.999	2.142	77.55	7.8
5	Svinelever	5mg	1.884	1.713	88.75	8.9
6	Svinelever	10mg	1.872	1.688	112.05	11.2

Elektroforesediagrammet er som følger:

Sag 3: A total RNA extraction experiment was conducted using multiple fresh tissue samples, og resultaterne er som følger:

Prøvenummer	Sample name	Sampling quantity	A260/A280	A260/A230	Konc.(ng/μL)	Produktion(μg)
1	Spinatblade	10mg	1.996	2.112	113.35	11.3
2	Chinese cabbage leaves	10mg	2.001	2.102	130.35	13
3	Bladselleri	10mg	1.999	2.142	77.55	7.8
4	Koriander blade	20mg	2.113	2.423	77.8	7.8
5	Koriander blade	20mg	2.03	2.116	203.75	20.4
6	Shiitake mushroom	10mg	2.171	2.68	65.8	6.6
7	Enoki mushrooms	10mg	2.073	1.969	44.45	4.5
8	White jade mushroom	20mg	2.157	2.517	56.8	5.7
9	Hvid champignon	20mg	2.111	2.42	118.7	11.9
10	Svinekød	10mg	2.041	2.011	32.2	3.2
11	Svinelever	5mg	1.884	1.713	88.75	8.9
12	Svinelever	10mg	1.872	1.708	112.05	11.2

Elektroforesediagrammet er som følger:

3.Experimental pain points

The universal total RNA extraction reagent (magnetisk perlemetode) has solved multiple experimental pain points in traditional RNA extraction by optimizing the technology and process, and is particularly suitable for complex samples and high-throughput requirements. The following are its core advantages and the corresponding solutions to pain points:

4.Product advantages

4.1 It has strong versatility and is suitable for extracting total RNA from various tissue samples such as animals, planter, svampe, and cells.

4.2 It is applicable to the extraction of total RNA from trace samples, trace tissue samples and cultured cells (a small number of cells) prøver.

4.3 The operation is simple and convenient. The magnetic bead method can be combined with an automatic nucleic acid extractor to achieve automated extraction. Lysis and lysis can be carried out in one step, making the extraction highly efficient.

4.4 No need for repeated centrifugation, at sikre integriteten af ​​det ekstraherede RNA.

Leverandør

Shanghai Lingjun Biotechnology Co., Ltd.blev etableret i 2016 som er en professionel producent af biomagnetiske materialer og nukleinsyreekstraktionsreagenser.

Vi har stor erfaring med udvinding og oprensning af nukleinsyre, proteinoprensning, celleadskillelse, kemiluminescens, og andre tekniske områder.

Vores produkter er meget udbredt inden for mange områder, såsom medicinsk test, genetisk testning, universitetsforskning, genetisk avl, og så videre. Vi leverer ikke kun produkter, men kan også påtage os OEM, ODM, og andre behov. Hvis du har et relateret behov, er du velkommen til at kontakte os .