Kit universal de extracción de ARN total: La clave para prevenir la degradación del ARN en experimentos de rutina

1. Introducción del producto:

The universal total RNA extraction reagent is suitable for rapidly and efficiently extracting total RNA from various tissue samples such as fresh plants, animals, hongos, and cells. The entire process is easy to operate and is particularly suitable for automated nucleic acid extraction platforms. During the extraction process, the sample releases nucleic acids under the action of the lysis buffer. The nano-magnetic beads selectively adsorb the nucleic acids under the action of the binding buffer, separar los ácidos nucleicos de otras impurezas en la muestra. Then rinse with the washing solution to remove proteins, polisacáridos, iones de sal inorgánica, etc.. Finalmente, high-quality RNA was obtained through elution. Este producto no requiere reactivos tóxicos como fenol y cloroformo.. The extracted RNA has a high yield, alta pureza, calidad estable y confiable, y se puede aplicar a varios experimentos de biología molecular posteriores..

2.Product case

Caso 1: RNA extraction experiments were conducted on multiple fresh tissue samples, y los resultados son los siguientes:

Número de muestra	Sample name	Sampling quantity	A260/A280	A260/A230	Conc.(ng/μL)	Producción(μg)
1	Baby cabbage leaves	10mg	2.073	2.112	109.35	10.9
2	Romaine lettuce leaves		2.01	1.974	114.8	11.5
3	Chicken feather vegetable leaves		2.109	2.045	79.45	8
4	Pea seedlings		2.053	1.972	108.75	10.9

The electrophoresis diagram is as follows:

Caso 2: A total RNA extraction experiment was conducted using multiple fresh tissue samples, y los resultados son los siguientes:

Número de muestra	Sample name	Sampling quantity	A260/A280	A260/A230	Conc.(ng/μL)	Producción(μg)
1	hojas de espinaca	10mg	1.996	2.112	113.35	11.3
2	hojas de cilantro		1.955	1.936	58.95	6
3	Chinese cabbage leaves		2.001	2.102	130.35	13
4	hojas de apio		1.999	2.142	77.55	7.8
5	hígado de cerdo	5mg	1.884	1.713	88.75	8.9
6	hígado de cerdo	10mg	1.872	1.688	112.05	11.2

The electrophoresis diagram is as follows:

Caso 3: A total RNA extraction experiment was conducted using multiple fresh tissue samples, y los resultados son los siguientes:

Número de muestra	Sample name	Sampling quantity	A260/A280	A260/A230	Conc.(ng/μL)	Producción(μg)
1	hojas de espinaca	10mg	1.996	2.112	113.35	11.3
2	Chinese cabbage leaves	10mg	2.001	2.102	130.35	13
3	hojas de apio	10mg	1.999	2.142	77.55	7.8
4	hojas de cilantro	20mg	2.113	2.423	77.8	7.8
5	hojas de cilantro	20mg	2.03	2.116	203.75	20.4
6	Shiitake mushroom	10mg	2.171	2.68	65.8	6.6
7	Enoki mushrooms	10mg	2.073	1.969	44.45	4.5
8	White jade mushroom	20mg	2.157	2.517	56.8	5.7
9	Hongo blanco	20mg	2.111	2.42	118.7	11.9
10	Cerdo	10mg	2.041	2.011	32.2	3.2
11	hígado de cerdo	5mg	1.884	1.713	88.75	8.9
12	hígado de cerdo	10mg	1.872	1.708	112.05	11.2

The electrophoresis diagram is as follows:

3.Experimental pain points

The universal total RNA extraction reagent (método de cuentas magnéticas) has solved multiple experimental pain points in traditional RNA extraction by optimizing the technology and process, and is particularly suitable for complex samples and high-throughput requirements. The following are its core advantages and the corresponding solutions to pain points:

4.Product advantages

4.1 It has strong versatility and is suitable for extracting total RNA from various tissue samples such as animals, plantas, hongos, and cells.

4.2 It is applicable to the extraction of total RNA from trace samples, trace tissue samples and cultured cells (a small number of cells) muestras.

4.3 The operation is simple and convenient. The magnetic bead method can be combined with an automatic nucleic acid extractor to achieve automated extraction. Lysis and lysis can be carried out in one step, making the extraction highly efficient.

4.4 No need for repeated centrifugation, asegurando la integridad del ARN extraído.

Proveedor

Shanghai Lingjun Biotecnología Co., Limitado.se estableció en 2016 que es un fabricante profesional de materiales biomagnéticos y reactivos de extracción de ácidos nucleicos..

Tenemos una rica experiencia en extracción y purificación de ácidos nucleicos., purificación de proteínas, separación celular, quimioluminiscencia, y otros campos técnicos.

Nuestros productos son ampliamente utilizados en muchos campos., como pruebas médicas, pruebas genéticas, investigación universitaria, mejoramiento genético, etcétera. No solo proporcionamos productos sino que también podemos realizar OEM, ODM, y otras necesidades. Si tienes una necesidad relacionada, no dude en contactarnos .