Universal Total RNA Extraction Kit: The Key to Preventing RNA Degradation in Routine Experiments

1. Product Introduction:

The universal total RNA extraction reagent is suitable for rapidly and efficiently extracting total RNA from various tissue samples such as fresh plants, animals, fungi, and cells. The entire process is easy to operate and is particularly suitable for automated nucleic acid extraction platforms. During the extraction process, the sample releases nucleic acids under the action of the lysis buffer. The nano-magnetic beads selectively adsorb the nucleic acids under the action of the binding buffer, separating the nucleic acids from other impurities in the sample. Then rinse with the washing solution to remove proteins, polysaccharides, inorganic salt ions, etc. Finally, high-quality RNA was obtained through elution. This product does not require toxic reagents such as phenol and chloroform. The extracted RNA has a high yield, high purity, stable and reliable quality, and can be applied to various downstream molecular biology experiments.

2.Product case

Case 1: RNA extraction experiments were conducted on multiple fresh tissue samples, and the results are as follows:

Sample number	Sample name	Sampling quantity	A260/A280	A260/A230	Conc. (ng/μL)	Output（μg）
1	Baby cabbage leaves	10mg	2.073	2.112	109.35	10.9
2	Romaine lettuce leaves		2.01	1.974	114.8	11.5
3	Chicken feather vegetable leaves		2.109	2.045	79.45	8
4	Pea seedlings		2.053	1.972	108.75	10.9

The electrophoresis diagram is as follows:

Case 2: A total RNA extraction experiment was conducted using multiple fresh tissue samples, and the results are as follows:

Sample number	Sample name	Sampling quantity	A260/A280	A260/A230	Conc. (ng/μL)	Output（μg）
1	Spinach leaves	10mg	1.996	2.112	113.35	11.3
2	Coriander leaves		1.955	1.936	58.95	6
3	Chinese cabbage leaves		2.001	2.102	130.35	13
4	Celery leaves		1.999	2.142	77.55	7.8
5	Pork liver	5mg	1.884	1.713	88.75	8.9
6	Pork liver	10mg	1.872	1.688	112.05	11.2

The electrophoresis diagram is as follows:

Case 3: A total RNA extraction experiment was conducted using multiple fresh tissue samples, and the results are as follows:

Sample number	Sample name	Sampling quantity	A260/A280	A260/A230	Conc. (ng/μL)	Output（μg）
1	Spinach leaves	10mg	1.996	2.112	113.35	11.3
2	Chinese cabbage leaves	10mg	2.001	2.102	130.35	13
3	Celery leaves	10mg	1.999	2.142	77.55	7.8
4	Coriander leaves	20mg	2.113	2.423	77.8	7.8
5	Coriander leaves	20mg	2.03	2.116	203.75	20.4
6	Shiitake mushroom	10mg	2.171	2.68	65.8	6.6
7	Enoki mushrooms	10mg	2.073	1.969	44.45	4.5
8	White jade mushroom	20mg	2.157	2.517	56.8	5.7
9	White mushroom	20mg	2.111	2.42	118.7	11.9
10	Pork	10mg	2.041	2.011	32.2	3.2
11	Pork liver	5mg	1.884	1.713	88.75	8.9
12	Pork liver	10mg	1.872	1.708	112.05	11.2

The electrophoresis diagram is as follows:

3.Experimental pain points

The universal total RNA extraction reagent (magnetic bead method) has solved multiple experimental pain points in traditional RNA extraction by optimizing the technology and process, and is particularly suitable for complex samples and high-throughput requirements. The following are its core advantages and the corresponding solutions to pain points:

4.Product advantages

4.1 It has strong versatility and is suitable for extracting total RNA from various tissue samples such as animals, plants, fungi, and cells.

4.2 It is applicable to the extraction of total RNA from trace samples, trace tissue samples and cultured cells (a small number of cells) samples.

4.3 The operation is simple and convenient. The magnetic bead method can be combined with an automatic nucleic acid extractor to achieve automated extraction. Lysis and lysis can be carried out in one step, making the extraction highly efficient.

4.4 No need for repeated centrifugation, ensuring the integrity of the extracted RNA.

Supplier

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 which is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence, and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but also can undertake OEM, ODM, and other needs. If you have a related need, please feel free to contact us .