Proteinase K

Proteinase K adalah protease yang sangat aktif dari kelas kimotripsin, dimurnikan dari Candida albicans Limber (album tirtirachium lentur), used for the general degradation of proteins in biological samples. The enzyme has two ca2+ binding sites, which are at some distance from the active centre of the enzyme and are not directly related to the catalytic mechanism. Namun, if ca2+ is removed from the enzyme, tentang 80% of the catalytic activity is lost due to the remote structural changes that occur, but its remaining activity is usually sufficient to degrade proteins that contaminate acid products in general.

Proteinase K adalah protease serin dengan aktivitas pembelahan luas. It cleaves the carboxy-terminal peptide bonds of aliphatic and aromatic amino acids. Ini aktif pada rentang pH yang luas (pH 4-12.5), is stable in urea and SDS, and has the ability to degrade natural proteins.

Specification

100mg regular or customised

Specific activity value：38 kamu/mg

Protein Source：Saccharomyces cerevisiae

Molecular mass：28,650

Aplikasi

Proteinase K has a wide range of uses, the main applications include: medical and basic research fields; genetic diagnostic kits; kit ekstraksi DNA genom; RNA extraction kits to remove the nuclease in the preparation of DNA and RNA; Selain itu, it is also used in the leather industry (leather softening and refining, menghilangkan dan melembutkan rambut, silk degelatinisation) the preparation of chromosomal DNA for pulse electrophoresis; the extraction of non-protein components in tissues; food and brewing industries: meat tenderisation, wine clarification; washing industry: enzyme laundry detergent, laundry detergent, dll.. are also used. Selain itu, it is also used in: leather industry (leather softening and refinement, menghilangkan dan melembutkan rambut, silk degumming); preparation of chromosomal DNA for pulse electrophoresis; extraction of non-protein components from tissues, degradation of protein-containing impurities; food and brewing industry: meat tenderisation, clarification of wines; and in the washing industry: enzyme-containing laundry detergents and liquid detergents.

Storage and use

The expiry date is 24 months under dry powder conditions and 12 months at -20°C in liquid form. Customers need to store at -20°C after dissolving with buffer. After dissolving, it is better to divide into several portions for convenience and practicality to avoid repeated freezing and thawing. If it needs to be placed in 2℃-8℃ environment, after more than 3 hari, dianjurkan untuk menyaring dan mendekontaminasi atau menambahkan zat bakteriostatik seperti 0.3% azida Na untuk mencegah kontaminasi mikroba.

Dilution Buffer

Larutkan hingga konsentrasi kerja 20mg/ml menggunakan buffer Tris-HCL 20mM (pH 7.5) sesuai kebutuhan.

Pengenalan Pemasok

Shanghai Lingjun Bioteknologi Co., Ltd.didirikan pada 2016 dan merupakan produsen profesional bahan biomagnetik dan kit ekstraksi asam nukleat.

Kami memiliki pengalaman yang kaya dalam ekstraksi dan pemurnian asam nukleat, pemurnian protein, pemisahan sel, chemiluminescence dan bidang teknis lainnya.

Produk kami banyak digunakan di berbagai bidang, misalnya tes kesehatan, pengujian genetik, penelitian universitas, pemuliaan genetik, dan sebagainya. Kami tidak hanya menyediakan produk tetapi juga dapat melakukan OEM, ODM, dan kebutuhan lainnya. Jika Anda memiliki kebutuhan terkait, jangan ragu untuk menghubungi kami dipenjualan01@lingjunbio.com.