プロテイナーゼK

Proteinase K is a highly active protease of the chymotrypsin class, カンジダ・アルビカンス・リンバーから精製 (tirtirachium album limber), used for the general degradation of proteins in biological samples. The enzyme has two ca2+ binding sites, which are at some distance from the active centre of the enzyme and are not directly related to the catalytic mechanism. しかし, if ca2+ is removed from the enzyme, about 80% of the catalytic activity is lost due to the remote structural changes that occur, but its remaining activity is usually sufficient to degrade proteins that contaminate acid products in general.

Proteinase K is a serine protease with broad cleavage activity. It cleaves the carboxy-terminal peptide bonds of aliphatic and aromatic amino acids. 広いpH範囲で活性を示します (pH 4-12.5), is stable in urea and SDS, and has the ability to degrade natural proteins.

仕様

100mg regular or customised

Specific activity value：38 U/mg

Protein Source：Saccharomyces cerevisiae

Molecular mass：28,650

応用

Proteinase K has a wide range of uses, the main applications include: medical and basic research fields; genetic diagnostic kits; ゲノム DNA 抽出キット; RNA extraction kits to remove the nuclease in the preparation of DNA and RNA; 加えて, it is also used in the leather industry (leather softening and refining, 脱毛と軟化, silk degelatinisation) the preparation of chromosomal DNA for pulse electrophoresis; the extraction of non-protein components in tissues; food and brewing industries: meat tenderisation, wine clarification; washing industry: enzyme laundry detergent, laundry detergent, 等. are also used. 加えて, it is also used in: leather industry (leather softening and refinement, 脱毛と軟化, silk degumming); preparation of chromosomal DNA for pulse electrophoresis; extraction of non-protein components from tissues, degradation of protein-containing impurities; food and brewing industry: meat tenderisation, clarification of wines; and in the washing industry: enzyme-containing laundry detergents and liquid detergents.

Storage and use

The expiry date is 24 months under dry powder conditions and 12 months at -20°C in liquid form. Customers need to store at -20°C after dissolving with buffer. After dissolving, it is better to divide into several portions for convenience and practicality to avoid repeated freezing and thawing. If it needs to be placed in 2℃-8℃ environment, after more than 3 日, 濾過して除染するか、次のような静菌剤を添加することをお勧めします。 0.3% 微生物汚染を防ぐアジ化Na.

Dilution Buffer

20mM Tris-HCL 緩衝液を使用して 20mg/ml の作業濃度まで溶解します。 (pH 7.5) 必要に応じて.

サプライヤー紹介

上海霊軍生物技術有限公司, 株式会社に設立されました 2016 生体磁性材料と核酸抽出キットの専門メーカーです.

核酸抽出・精製の経験が豊富です。, タンパク質の精製, 細胞分離, 化学発光およびその他の技術分野.

当社の製品はさまざまな分野で広く使用されています, 医療検査など, 遺伝子検査, 大学の研究, 遺伝子育種, 等々. 製品のご提供だけでなく、OEMも承ります。, ODM, その他のニーズ. 関連するニーズがある場合, お気軽にお問い合わせください。sales01@lingjunbio.com.