Proteinase K

Proteinase K ass eng héich aktiv Protease vun der Chymotrypsin Klass, aus Candida albicans Limber gereinegt (tirtirachium album limber), used for the general degradation of proteins in biological samples. The enzyme has two ca2+ binding sites, which are at some distance from the active centre of the enzyme and are not directly related to the catalytic mechanism. Allerdéngs, if ca2+ is removed from the enzyme, iwwer 80% of the catalytic activity is lost due to the remote structural changes that occur, but its remaining activity is usually sufficient to degrade proteins that contaminate acid products in general.

Proteinase K ass eng Serinprotease mat breet Spaltungsaktivitéit. It cleaves the carboxy-terminal peptide bonds of aliphatic and aromatic amino acids. Et ass aktiv iwwer eng breet pH-Beräich (pH 4-12.5), is stable in urea and SDS, and has the ability to degrade natural proteins.

Spezifizéierung

100mg regular or customised

Specific activity value：38 U/mg

Protein Source：Saccharomyces cerevisiae

Molecular mass：28,650

Applikatioun

Proteinase K has a wide range of uses, the main applications include: medical and basic research fields; genetic diagnostic kits; genomesch DNA Extraktioun Kits; RNA extraction kits to remove the nuclease in the preparation of DNA and RNA; Zousätzlech, it is also used in the leather industry (leather softening and refining, Entfernung an Erweichung, silk degelatinisation) the preparation of chromosomal DNA for pulse electrophoresis; the extraction of non-protein components in tissues; food and brewing industries: meat tenderisation, wine clarification; washing industry: enzyme laundry detergent, laundry detergent, etc. are also used. Zousätzlech, it is also used in: leather industry (leather softening and refinement, Entfernung an Erweichung, silk degumming); preparation of chromosomal DNA for pulse electrophoresis; extraction of non-protein components from tissues, degradation of protein-containing impurities; food and brewing industry: meat tenderisation, clarification of wines; and in the washing industry: enzyme-containing laundry detergents and liquid detergents.

Storage and use

The expiry date is 24 months under dry powder conditions and 12 months at -20°C in liquid form. Customers need to store at -20°C after dissolving with buffer. After dissolving, it is better to divide into several portions for convenience and practicality to avoid repeated freezing and thawing. If it needs to be placed in 2℃-8℃ environment, after more than 3 Deeg, et ass recommandéiert ze filteren an ze dekontaminéieren oder bakteriostatescht Agent z.B 0.3% Azid Na fir mikrobiell Kontaminatioun ze vermeiden.

Dilution Buffer

Opléisen zu enger Aarbechtskonzentratioun vun 20mg/ml mat 20mM Tris-HCL Puffer (pH 7.5) wéi néideg.

Fournisseur Aféierung

Shanghai Lingjun Biotechnology Co., Ltd., Ltd.gouf etabléiert an 2016 an ass e professionnelle Hiersteller vu biomagnetesche Materialien an Nukleinsäure Extraktioun Kit.

Mir hu räich Erfahrung an Nukleinsäure Extraktioun an Offäll, Protein Reinigung, Zell Trennung, Chemilumineszenz an aner technesch Felder.

Eis Produkter gi wäit a ville Beräicher benotzt, wéi medizinesch Tester, genetesch Tester, Universitéit Fuerschung, genetesch Zucht, an esou weider. Mir liwweren net nëmmen Produkter awer kënnen och OEM maachen, ODM, an aner Besoinen. Wann Dir verbonne Besoinen hutt, weg fillen gratis eis ze kontaktéieren umsales01@lingjunbio.com.