단백질분해효소 K

Proteinase K는 키모트립신 계열의 매우 활성이 높은 프로테아제입니다., 칸디다 알비칸스 림버(Candida albicans Limber)로부터 정제됨 (티티라치움 앨범 림버), used for the general degradation of proteins in biological samples. The enzyme has two ca2+ binding sites, which are at some distance from the active centre of the enzyme and are not directly related to the catalytic mechanism. 하지만, if ca2+ is removed from the enzyme, ~에 대한 80% of the catalytic activity is lost due to the remote structural changes that occur, but its remaining activity is usually sufficient to degrade proteins that contaminate acid products in general.

Proteinase K는 광범위한 절단 활성을 갖는 세린 프로테아제입니다.. It cleaves the carboxy-terminal peptide bonds of aliphatic and aromatic amino acids. 넓은 pH 범위에서 활성화됩니다. (pH 4-12.5), is stable in urea and SDS, and has the ability to degrade natural proteins.

사양

100mg regular or customised

Specific activity value：38 U/mg

Protein Source：Saccharomyces cerevisiae

Molecular mass：28,650

애플리케이션

Proteinase K has a wide range of uses, the main applications include: medical and basic research fields; genetic diagnostic kits; 게놈 DNA 추출 키트; RNA extraction kits to remove the nuclease in the preparation of DNA and RNA; 게다가, it is also used in the leather industry (leather softening and refining, 제모 및 연화, silk degelatinisation) the preparation of chromosomal DNA for pulse electrophoresis; the extraction of non-protein components in tissues; food and brewing industries: meat tenderisation, wine clarification; washing industry: enzyme laundry detergent, laundry detergent, 등. are also used. 게다가, it is also used in: leather industry (leather softening and refinement, 제모 및 연화, silk degumming); preparation of chromosomal DNA for pulse electrophoresis; extraction of non-protein components from tissues, degradation of protein-containing impurities; food and brewing industry: meat tenderisation, clarification of wines; and in the washing industry: enzyme-containing laundry detergents and liquid detergents.

Storage and use

The expiry date is 24 months under dry powder conditions and 12 months at -20°C in liquid form. Customers need to store at -20°C after dissolving with buffer. After dissolving, it is better to divide into several portions for convenience and practicality to avoid repeated freezing and thawing. If it needs to be placed in 2℃-8℃ environment, after more than 3 날, 여과하고 오염을 제거하거나 다음과 같은 정균제를 첨가하는 것이 좋습니다. 0.3% 미생물 오염을 방지하는 azide Na.

Dilution Buffer

20mM Tris-HCL 완충액을 사용하여 20mg/ml의 작업 농도로 용해합니다. (pH 7.5) 필요에 따라.

공급업체 소개

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