புரோட்டினேஸ் கே

Proteinase K is a highly active protease of the chymotrypsin class, purified from Candida albicans Limber (tirtirachium album limber), used for the general degradation of proteins in biological samples. The enzyme has two ca2+ binding sites, which are at some distance from the active centre of the enzyme and are not directly related to the catalytic mechanism. எனினும், if ca2+ is removed from the enzyme, about 80% of the catalytic activity is lost due to the remote structural changes that occur, but its remaining activity is usually sufficient to degrade proteins that contaminate acid products in general.

Proteinase K is a serine protease with broad cleavage activity. It cleaves the carboxy-terminal peptide bonds of aliphatic and aromatic amino acids. It is active over a wide pH range (pH 4-12.5), is stable in urea and SDS, and has the ability to degrade natural proteins.

Specification

100mg regular or customised

Specific activity value：38 U/mg

Protein Source：Saccharomyces cerevisiae

Molecular mass：28,650

விண்ணப்பம்

Proteinase K has a wide range of uses, the main applications include: medical and basic research fields; genetic diagnostic kits; genomic DNA extraction kits; RNA extraction kits to remove the nuclease in the preparation of DNA and RNA; In addition, it is also used in the leather industry (leather softening and refining, hair removal and softening, silk degelatinisation) the preparation of chromosomal DNA for pulse electrophoresis; the extraction of non-protein components in tissues; food and brewing industries: meat tenderisation, wine clarification; washing industry: enzyme laundry detergent, laundry detergent, முதலியன. are also used. In addition, it is also used in: leather industry (leather softening and refinement, hair removal and softening, silk degumming); preparation of chromosomal DNA for pulse electrophoresis; extraction of non-protein components from tissues, degradation of protein-containing impurities; food and brewing industry: meat tenderisation, clarification of wines; and in the washing industry: enzyme-containing laundry detergents and liquid detergents.

Storage and use

The expiry date is 24 months under dry powder conditions and 12 months at -20°C in liquid form. Customers need to store at -20°C after dissolving with buffer. After dissolving, it is better to divide into several portions for convenience and practicality to avoid repeated freezing and thawing. If it needs to be placed in 2℃-8℃ environment, after more than 3 days, it is recommended to filter and decontaminate or add bacteriostatic agent such as 0.3% azide Na to prevent microbial contamination.

Dilution Buffer

Dissolve to a working concentration of 20mg/ml using 20mM Tris-HCL buffer (pH 7.5) as required.

சப்ளையர் அறிமுகம்

ஷாங்காய் லிங்ஜுன் பயோடெக்னாலஜி கோ., லிமிடெட்இல் நிறுவப்பட்டது 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction kit.

நியூக்ளிக் அமிலம் பிரித்தெடுத்தல் மற்றும் சுத்திகரிப்பு ஆகியவற்றில் எங்களுக்கு சிறந்த அனுபவம் உள்ளது, புரத சுத்திகரிப்பு, செல் பிரிப்பு, வேதியியல் மற்றும் பிற தொழில்நுட்ப துறைகள்.

எங்கள் தயாரிப்புகள் பல துறைகளில் பரவலாகப் பயன்படுத்தப்படுகின்றன, மருத்துவ பரிசோதனை போன்றவை, மரபணு சோதனை, பல்கலைக்கழக ஆராய்ச்சி, மரபணு இனப்பெருக்கம், மற்றும் பல. நாங்கள் தயாரிப்புகளை வழங்குவது மட்டுமல்லாமல் OEM ஐயும் மேற்கொள்ள முடியும், ODM, மற்றும் பிற தேவைகள். உங்களுக்கு தொடர்புடைய தேவைகள் இருந்தால், தயவு செய்து எங்களை தொடர்பு கொள்ளவும்sales01@lingjunbio.com.