Proteinase K

Proteinase K adalah protease yang sangat aktif dari kelas chymotrypsin, disucikan daripada Candida albicans Limber (album titirachium limber), used for the general degradation of proteins in biological samples. The enzyme has two ca2+ binding sites, which are at some distance from the active centre of the enzyme and are not directly related to the catalytic mechanism. Namun begitu, if ca2+ is removed from the enzyme, kira-kira 80% of the catalytic activity is lost due to the remote structural changes that occur, but its remaining activity is usually sufficient to degrade proteins that contaminate acid products in general.

Proteinase K ialah protease serin dengan aktiviti pembelahan yang luas. It cleaves the carboxy-terminal peptide bonds of aliphatic and aromatic amino acids. Ia aktif dalam julat pH yang luas (pH 4-12.5), is stable in urea and SDS, and has the ability to degrade natural proteins.

Spesifikasi

100mg regular or customised

Specific activity value：38 U/mg

Protein Source：Saccharomyces cerevisiae

Molecular mass：28,650

Permohonan

Proteinase K has a wide range of uses, the main applications include: medical and basic research fields; genetic diagnostic kits; genomic DNA extraction kits; RNA extraction kits to remove the nuclease in the preparation of DNA and RNA; Selain itu, it is also used in the leather industry (leather softening and refining, hair removal and softening, silk degelatinisation) the preparation of chromosomal DNA for pulse electrophoresis; the extraction of non-protein components in tissues; food and brewing industries: meat tenderisation, wine clarification; washing industry: enzyme laundry detergent, laundry detergent, dll. are also used. Selain itu, it is also used in: leather industry (leather softening and refinement, hair removal and softening, silk degumming); preparation of chromosomal DNA for pulse electrophoresis; extraction of non-protein components from tissues, degradation of protein-containing impurities; food and brewing industry: meat tenderisation, clarification of wines; and in the washing industry: enzyme-containing laundry detergents and liquid detergents.

Storage and use

The expiry date is 24 months under dry powder conditions and 12 months at -20°C in liquid form. Customers need to store at -20°C after dissolving with buffer. After dissolving, it is better to divide into several portions for convenience and practicality to avoid repeated freezing and thawing. If it needs to be placed in 2℃-8℃ environment, after more than 3 hari, adalah disyorkan untuk menapis dan menyahcemar atau menambah agen bakteriostatik seperti 0.3% azide Na untuk mengelakkan pencemaran mikrob.

Dilution Buffer

Larutkan kepada kepekatan kerja 20mg/ml menggunakan penimbal Tris-HCL 20mM (pH 7.5) mengikut keperluan.

Pengenalan Pembekal

Shanghai Lingjun Biotechnology Co., Ltd.ditubuhkan pada 2016 dan merupakan pengilang profesional bahan biomagnet dan kit pengekstrakan asid nukleik.

Kami mempunyai pengalaman yang kaya dalam pengekstrakan dan penulenan asid nukleik, penulenan protein, pemisahan sel, chemiluminescence dan bidang teknikal lain.

Produk kami digunakan secara meluas dalam pelbagai bidang, seperti ujian perubatan, ujian genetik, penyelidikan universiti, pembiakan genetik, dan seterusnya. Kami bukan sahaja menyediakan produk tetapi juga boleh menjalankan OEM, ODM, dan keperluan lain. Jika anda mempunyai keperluan yang berkaitan, sila hubungi kami disales01@lingjunbio.com.