효율적인 RNA 추출 가이드: 원칙부터 실천까지, 고품질 RNA 분리 보장

Introduction of RNA 추출

RNA extraction is a basic step in molecular biology and gene expression research. 그 목적은 그대로 분리하는 것입니다., 분해되지 않은 RNA (리보핵산) from cell or tissue samples. RNA participates in a variety of biological processes, such as transcription of genetic information and protein synthesis in cells. 그러므로, obtaining high-quality RNA is crucial for subsequent molecular biological analysis, such as RT-PCR, Northern Blot, RNA sequencing, 등.

Principle of RNA Extraction

Denaturants break cells or tissues, and then RNA is extracted by organic solvents such as chloroform and then precipitated, washed, dried, and finally dissolved.

하지만, since RNA enzymes are everywhere and may degrade RNA at any time, there are many places to pay attention to in the experiment. Any negligence will fail.

Avoid detours in RNA extraction

How to choose the most suitable RNA extraction reagent for different materials.

The numerous existing RNA isolation methods may make it difficult to choose. 현재, the simplest and safest method is column separation, such as RNApure, which is popular because of its simple operation, short time and high purity. RNApure does not require DNase to digest DNA, which saves time and avoids RNA degradation, thereby increasing the yield; compared with non-column separation (such as TRIzol), it removes proteins and other impurities cleanly, improves purity, and is very suitable for cells, 조직, and general plants. The extraction of plant RNA is more difficult to choose. Plant RNA extraction is affected by the content of phenol, 다당류, protein impurities, 및 2차 대사산물. 일반적으로, the purity of TRIzol extraction is not high, and many plants cannot be extracted with TRIzol.

이때, you can choose polysaccharide and polyphenol plant total RNA extraction kit (daffodils, peppers, carrots, 옥수수, lilies, 밀, tomatoes, cauliflower, rapeseed, 등.) and universal plant RNA extraction kit (suitable for most plant extractions, including various Chinese herbal medicines, plant seeds, apples, grapes, 딸기, bananas, longans, litchi, lawn plants, pine trees, fir trees, birch trees, purple pine cones, coleus, poinsettia, oleander, fig, violet, rose, dahlia, morning glory, 등.); It is worth mentioning that the extraction of RNA from blood (혈청을 포함하여, 혈장, 뇌척수액, various swabs or other samples with high liquid content) using TRIzol and red blood cell lysis methods are not effective because red blood cell lysis buffer does not contain RNase inhibitors, and RNA is easily degraded in this process. It is recommended to use TRIpure LS (RP1101) or a blood total RNA extraction kit (RP4001). This method is suitable for RNA extraction in expression profile chip experiments and has become a nationally recommended method.

How to store extracted RNA?

If it is only for short-term storage, the resuspended RNA should be placed at -20°C; if it is for long-term storage, it should be placed at -80°C. When storing RNA, a small amount of RNase inhibitor (RP5601) can be added to avoid RNA degradation, and RNA can be directly used for downstream experiments. If RNA is to be stored for a long time, RNAlong can be added. We recommend that the RNA solution be divided into several tubes. This will avoid repeated freezing and thawing to damage the RNA and prevent accidental RNase contamination.

공급자

상하이 링쥔 생명공학 유한회사, 주식회사. 에 설립되었습니다 2016 생체자성재료 및 핵산추출시약 전문제조업체입니다..

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