Почему все больше преподавателей колледжей используют метод магнитных шариков вместо традиционного??

В последние годы, Технология экстракции нуклеиновых кислот на основе магнитных шариков has been rapidly popularized in universities and research institutions, постепенно заменяя традиционные методы фенол-хлороформной экстракции и методы центробежной колонки.. This is mainly attributed to its high efficiency, potential for automation, безопасность, and adaptability to complex samples. The following is the specific analysis of the reasons:

1. Easy to operate and compatible with high-throughput automation

Pain points of traditional methods

Phenol-chloroform method: It requires multiple centrifugations and phase separations, is cumbersome to operate and prone to contamination.

Метод центробежной колонки: It involves many manual steps (such as lysis, column passing, стирка, и т. д.) and has a low throughput.

Advantages of the magnetic bead method

After the magnetic beads bind to nucleic acids, they can be rapidly separated through a magnetic field (without the need for centrifugation) and directly integrated into a 96-well plate automated platform, который подходит для крупномасштабной обработки образцов (such as epidemiological screening and omics research).

Случай: During the COVID-19 pandemic, the magnetic bead method became the preferred choice for high-throughput nucleic acid testing.

2. Higher extraction efficiency and purity

Strong binding specificity

Silicon-based magnetic beads selectively adsorb nucleic acids under high-salt conditions, reducing protein and polysaccharide contamination (the traditional phenol-chloroform method is prone to residual organic solvents, and the centrifugal column method is prone to clogging).

High recovery rate

Magnetic beads have a large surface area, and their capture efficiency for small DNA/RNA fragments and low-concentration samples is superior to that of centrifugal columns (например, the recovery rate of cfDNA extraction is increased by 20% к 30%).

Data support

Literature reports that the A260/A230 ratio of plant RNA extracted by magnetic bead method is generally greater than 2.0, while the traditional method often leads to a ratio of less than 1.5 due to polysaccharide residue.

3. Wider sample applicability

Complex sample processing capability

The magnetic bead method can be adapted to difficult-to-extract samples such as plant tissues, фекалии, земля, and FFPE sections by adjusting the buffer solution (such as adding CTAB or PVPP), while the centrifugal column method is prone to clogging.

Flexibly respond to trace samples

Magnetic beads can bind to trace amounts of nucleic acids (such as Pg-level DNA in single-cell sequencing), but the loss rate of traditional methods is high.

4. Safety and environmental protection advantages

Avoid toxic reagents

The magnetic bead method does not require carcinogens such as phenol and chloroform, reducing the health risks to laboratory personnel.

Reduce plastic waste

The centrifugal column method consumes a large amount of plastic consumables, while the magnetic bead method can reuse magnetic beads (например, some models can be recycled 35 раз), which is more environmentally friendly.

5. Optimization of cost-benefit ratio

High initial cost, more economical in the long term

Although the unit price of magnetic bead reagents is high, automation can reduce labor costs and is especially suitable for large-scale projects.

University case: A laboratory’s comparison found that when processing 1,000 образцы, the total cost of the magnetic bead method was 15% к 20% lower than that of the centrifugal column method.

Acceleration of domestic substitution

Домашние магнитные бусины (such as Nanomicro and Borui) have performance close to imported brands, but their prices are only 1/3 к 1/2, which promotes their popularization.

6. Technology Integration and cutting-edge applications

Compatible molecular diagnostic technology

The nucleic acids extracted by the magnetic bead method can be directly used for qPCR, NGS and CRISPR detection without additional purification.

Demand in emerging fields

Single-cell sequencing, жидкая биопсия (ктДНК), and other methods that require high-purity nucleic acids, the magnetic bead method is the mainstream choice.

The selection logic of universities/research institutions

1. Efficiency-driven: The labor cost for postgraduate students is high, and automation liberates labor.

2. Data quality requirements: High-throughput sequencing and other methods require high-purity nucleic acids, and the magnetic bead method is more reliable.

3. Safety and Compliance: EHS (Environmental Health and Safety) management in colleges and universities is becoming stricter, and phenolic reagents are being phased out.

Будущие тенденции

The surface modification of magnetic beads is diverse: such as EPCAM-modified magnetic beads suitable for exosome extraction.

Microfluidic integration: The combination of magnetic bead method and Lab-on-a-chip to achieve “sample in, result out”.

Метод магнитных шариков, with its characteristics of “безопасность, efficiency and flexibility”, has become the mainstream choice for nucleic acid extraction. Especially in the context of the demand for complex samples and high throughput in scientific research scenarios, it is inevitable to replace traditional methods.

Поставщик

Шанхайская биотехнологическая компания Линцзюнь., ОООбыл создан в 2016 который является профессиональным производителем биомагнитных материалов и реагентов для экстракции нуклеиновых кислот..

Мы имеем богатый опыт в экстракции и очистке нуклеиновых кислот., очистка белка, разделение клеток, хемилюминесценция, и другие технические области.

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